While our manuscript was in preparation, an alternative approach was published to identify proteins co-modified by SUMO and ubiquitin (40). Thibault and co-workers employed a His6-SUMO3 purification and a seque
Kiihner and co- workers44. Moreover, during the segregation, polymersomes’ membrane became thicker at the protrusions and thinner everywhere else, keeping the structure stable since the thick-walled compartments exhibit larger spontaneous curvatures than the thin-walled compartment. Fig. 5: The ...
Many pathogenic viruses are surrounded by an envelope consisting of host cell membrane lipids and proteins, and viral glycoproteins. These proteins are the targets of neutralizing antibodies and essential components of a vaccine. Due to the inherent properties of the lipid envelope, assembly of envelop...
Socially exchanged fluids are a direct means by which an organism can influence conspecifics. It was recently shown that when workers of the carpenter antCamponotus floridanusfeed larval offspring via trophallaxis, they transfer Juvenile Hormone III (JH), a key developmental regulator, as well as ...
ScpA is the C-terminal extension of the ferrochelatase HemH, an enzyme involved in heme biosyn- thesis (Funk and Vermaas 1999; Sobotka et al. 2008, 2011; Storm et al. 2013); ScpB–E are proteins of around 6 kDa and have been found to be associated with PSII (Promnares et...
All workers have been trained by EHS to safely use powered air purifying respirators (PAPRs), and appropriate work habits in a BSL3 facility and active medical surveillance plans are in place. Our CoV BSL3 facilities contain redundant fans, emergency power to fans and biological safety cabinets...
Sharp pointed out that when the steric effects of macromolecular crowders and small molecules like water and ions are treated on an equal footing, the effect of the macromolecules are less effective at crowding than water and ions [60]. Shah and coworkers developed a molecular thermodynamic ...
proteins acting together in order to coordinate the M-phase progression [1–3]. Mutation of a protein playing a key role during chromosome separation would be expected to lead to cell death. However, when a protein has a loss-of-function mutation and the protein plays a subtle role in ...
In particular, Sapp and co-workers had demonstrated by immunoblotting that ˜50 percent of the L1 protein of HPV-33 VLPs was disulfide—bonded into a range of larger oligomers with an apparent Mr consistent with trimers of L1, and that mild reducing conditions partially broke down HPV-33 ...
(19, 26, 63). Interestingly, both our analysis and the recent modeling of Sulea and coworkers (53) identify an additional and conserved residue, D1826, which may also be important for PLpro activity (Fig. 2, boxed in red). We hypothesize that coronavirus papain-like proteases, like ...