This technique involves predicting the interaction between a small molecule and a protein at the atomic level1. This enables researchers to study the behavior of small molecules, such as nutraceuticals, within the binding site of a target protein and understand the fundamental biochemical process ...
Gene expression involves two major steps: transcription and translation (Fig.1a). Transcription is the process in which mRNA is synthesized by RNA polymerase using DNA as a template. The transcription rate of a geneitherefore depends on the number of RNA polymerasesnand its effective gene copy num...
Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible for cell reprogramming during somatic embryogenesis are still largely unclear. Recent advances in single-cell technol
the innate system. Its troops are called nk (natural killer) cells and macrophages, and one of the red flags it reacts to is an absence of any sort of hla. It can, however, be warded off by over-expression of a protein called cd47, something that Dr Schrepfer’s team also ...
For each gene of interest, qRT-PCR was performed as follows: each RNA sample was tested in duplicate and p0 (human and mouse) was used to normalize transcript abundance. mRNA expression levels were calculated by Comparative Ct Method by using the Applied Biosystem software (Applied Biosystem, ...
[88]. Kotta et al. utilized response surface methodology to optimize the formulation and process of a resveratrol nanoemulsion. In addition, they evaluated the in vitro drug release, in vitro permeation, and brain targeting properties of the nanoemulsion. Their findings demonstrated that the in ...
it remains unclear how the two potato cultivars, Q9 (moderately resistant) and Atl (susceptible), initiate distinct defense responses after inoculation withP. infestans. To uncover the underlying process, we investigated the dynamic gene expression profiles and the functions of the differentially express...
However, these methods capture a limited number of pre-defined molecular features among the affected cells. Another experimental approach involves using conventional RNA-seq to identify gene expression changes in the TME. However, standard RNA-seq requires processing the tissues in bulk and lacks the...
Gene expression involves transcription, translation and the turnover of mRNAs and proteins. The degree to which protein abundances scale with mRNA levels and the implications in cases where this dependency breaks down remain an intensely debated topic. Here we review recent mRNA–protein correlation st...
et al. Human haematopoietic stem cell lineage commitment is a continuous process. Nat. Cell Biol. 19, 271–281 (2017). Article CAS PubMed PubMed Central Google Scholar Zemmour, D. et al. Single-cell gene expression reveals a landscape of regulatory T cell phenotypes shaped by the TCR. ...