Real-time RT-PCR was performed with SYBR Green PCR Master Mix (Thermo Fisher Scientific) using a StepOnePlus real-time PCR system (Thermo Fisher Scientific). The relative quantitation of target mRNA levels was performed using the 2−ΔΔCT method. The values were normalized by those of the...
aPolymerase Chain Reaction (PCR) is used to screen samples for a specific genus or species. The PCR process is based on in-vitro replication of selected nucleic acid sequences. 聚合酶链式反应 (PCR) 用于筛选样品为一个具体类或种类。 PCR过程根据选择的核酸序列的体外复制。[translate]...
The PCR reaction were performed in 25 μL mixture containing 2.5 μL of 10× Buffer (with Mg2+), 0.5 μL of each universal primers, 1 μL of dNTP (2.5 mM), 15 μL of sterilized water, and 0.5 μL of genomic DNA. The PCR products were checked for the expected size on 1.0% ...
Define PCR reaction. PCR reaction synonyms, PCR reaction pronunciation, PCR reaction translation, English dictionary definition of PCR reaction. n. Abbr. PCR A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and inc
The ddPCR reaction was performed in a QX200 Droplet Digital PCR System (Bio-Rad) according to the manufacturer’s instructions. Each test was prepared in a total of 20-μl volume of the reaction mixture, comprising 10 μl of EvaGreen Supermix (Bio-Rad), 2 µl of forward and ...
PCR — The Basic ReactionNo Abstract available for this chapter.doi:10.1007/1-4020-3404-0_2Gerrit J. ViljoenLouis H. NelJohn R. CrowtherSpringer Netherlands
The polymerase chain reaction (PCR) is a primer-mediated enzymatic amplification of specifically cloned or genomic DNA sequences (1). This PCR process, invented more than a decade ago, has been automa Lori A Kolmodin,David E Birch - 《Methods in Molecular Biology》 被引量: 22发表: 2002年...
To label long ssDNA/dsDNA substrates internally, the [α-32P] dATP was directly added to the PCR reaction. The PCR product was agarose-gel-purified and kept at −20 °C in the form of dsDNA. On the day of the assay, the ssDNA substrate was made by diluting the dsDNA to the ...
The quantitative PCR experiments were performed on qTOWER2.0 (Analytic Jena, Germany). The real-time PCR reaction system contained 10 μL of SYBR Green Real-time PCR Master Mix (Takara), forward and reverse primers (0.5 μmol L−1 each), and 2 μL of cDNA or DNA as the ...
Polymerase Chain Reaction (PCR) analyses on gDNA and cDNA confirmed the presence of circPIAS1 in human and mouse melanoma cells (Fig. 1e). Furthermore, RNase R exonuclease tolerance experiments provided additional validation (Fig. 1f-g). Fluorescence in situ hybridization (FISH) analysis revealed...