Location and structure of the gene on yeast mitochondrial DNA: Nucleotide sequence of the alleleMichael E.S. HudspethW.Michael AinleyDeborah S. ShumardRonald A. Butowand Lawrence I. GrossmanCurrent Biology
The gene in is responsible for the isozyme conversion of . We analyzed the 1,664-nucleotide sequence of a chromosomal DNA segment that contained the gene and its flanking regions. The predicted product contained 345 amino acids with an estimated molecular weight of 37,919. The 24-amino-acid ...
Using nuclease S 1 mapping, the sole transcription startpoint for the nirB gene was found 23 or 24 base-pairs upstream from the ATG initiation codon. By subcloning successively smaller DNA fragments into a β-galactosidase expression vector plasmid, we located the promoter within a sequence ...
The envelope protein gene of the tick-borne flavivirus, Kumlinge A52, the prototype Finnish strain, has been amplified and sequenced.* The nucleotide and deduced amino acid sequence has been analyzed and compared with the closely related tick-borne encephalitis (TBE) virus, Western subtype, strai...
As such, we retrieved gene sequences targeted by the AHE probes for three fly species: Drosophila melanogaster, Phlebotomus papatasi, and Anopheles gambiae. These three species were used in the original AHE project to identify probe sequences in sequencing reads44. The nucleotide sequences for ...
Cytosine methylation changes at transcription factor binding sites could potentially alter the binding strength of transcription factors and decrease gene expression, while methylation in the gene body could increase transcript levels39. We used the Reference Sequence (RefSeq) database to annotate transcribe...
Some gene panels may include promoter sequences. a. Whole-genome sequencing Whole-genome sequencing, or WGS, is performed to sequence the entire genome of an organism using the total genomic DNA. WGS data of a sample is then...
Moreover, we have also identified several gene fragments from plastid migration, includingndhJ,ndhK,ndhC,rpl16, and so on. These gene fragments might have experienced sequence loss during the migration process. A schematic representation of the MTPTs is provided in Fig.4. ...
, with the 39 sequences picked for validation marked by an asterisk; the inner circle shows the SSN cluster of the corresponding sequence; the middle circle indicates the taxonomy of each sequence at the phylum level; the outermost circle shows the conserved domain organization of each sequence....
Gene expression involves transcription, translation and the turnover of mRNAs and proteins. The degree to which protein abundances scale with mRNA levels and the implications in cases where this dependency breaks down remain an intensely debated topic. H