Summary This chapter contains sections titled: Regulation of Gene Expression: How and Where? A Schematic Overview Protein-Nucleic Acid Interactions as a Basis for Specific Gene Regulation Structural Motifs of DN
The first reporter contains the MS2 hairpin 30 base pairs downstream of the 5′ splice site (lucMAPT-30D), and the second contains the MS2 hairpin 30 base pairs upstream of the 3′ splice site (lucMAPT-30U). The MS2 hairpin recruits MS2 coat protein (MCP) fused to RBP open reading ...
Repetitive DNA sequences playing critical roles in driving evolution, inducing variation, and regulating gene expression. In this review, we summarized the definition, arrangement, and structural characteristics of repeats. Besides, we introduced diverse biological functions of repeats and reviewed existing...
14. Specifically, representative sequences for fadA, bft, the pks genomic island, and the bai operon were identified from integrated gene catalog (IGC)60 using the gene-specific Hidden Markova Model. Subsequently, metagenomic sequencing reads were mapped to the IGC using bowtie2, and the read ...
resist the degradation effect of extracellular nucleases, making it a suitable carrier for small nucleic acid drugs [126,127]. Currently, there are three main types of EVs used for vector development in industry: human engineered exosomes, exosomes derived from human mesenchymal stem cells (MSCs),...
But the biggest hurdle—one not unique to siRNAs—was getting the nucleic acid payloads where they needed to go. Then, in 2014, Alnylam researchers and their academic colleagues reported that linking siRNAs with a ligand called GalNAc would get them into cells in the liver, but not into ...
et al. The Human Xist gene—analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus. Cell 71, 527–542 (1992). Article CAS PubMed Google Scholar Kickhoefer, V. A., Vasu, S. K. & Rome, L. H. Vaults are the answer,...
Maintenance of plasmids The pcDNA6/TR™ vector contains the ampicillin and blasticidin resistance genes to allow selection of the plasmid using ampicillin or blasticidin. To propagate and maintain the pcDNA6/TR plasmid, we recommend ...
ARF, Auxin response factor; CNR1, cell number regulator 1; DA (LARGE IN CHINESE) 1; FAD, fatty acid desaturase; GA20ox, gibberellin 20-oxidase; GA2ox, gibberellin 2-beta-dioxygenase; IAA, auxin-induced gene; NRT1, nitrate transporter 1; PROG1, prostrate growth 1; RMG1, resistance ...
Developmental plasticity describes the influence of environmental factors on phenotypic variation. An important mediator of developmental plasticity in many animals is parental care. Here, we examine the consequences of maternal care on offspring after t