The first reporter contains the MS2 hairpin 30 base pairs downstream of the 5′ splice site (lucMAPT-30D), and the second contains the MS2 hairpin 30 base pairs upstream of the 3′ splice site (lucMAPT-30U). The MS2 hairpin recruits MS2 coat protein (MCP) fused to RBP open reading ...
The percentage of homologous genes belonging to different expression groups was compared between intersubgenome homoeologous chromosomes. As the percentages of the two subgenomes did not show persistent differences in any expression group (Fig. 5c), we speculated that the gene expression also did not ...
The combined set of LC-MS/MS data and existing RelA-associated proteins contains 632 unique proteins. Within this group, the mRNA expression profiles are available for 620 of the proteins, and these were selected for further analysis (supplemental Data File S1, Sheet 5). Several factors may be...
Anthocyanins are the most important compounds for nutritional quality and economic values of blood orange. However, there are few reports on the pre-harvest treatment accelerating the accumulation of anthocyanins in postharvest blood orange fruit. Here,
Gene divergence could occur due to strong selection of these biotrophs. By comparison, two related biotrophic smut fungi from different genera, U. maydis and Sporisorium reilianum, still share an average 74% amino acid identity among predicted proteins [32]. A closer look at the set of ...
resist the degradation effect of extracellular nucleases, making it a suitable carrier for small nucleic acid drugs [126,127]. Currently, there are three main types of EVs used for vector development in industry: human engineered exosomes, exosomes derived from human mesenchymal stem cells (MSCs),...
Plant genome contains entire genetic information of a species, recording its evolutionary history and direction. Comprehensive understanding of plant genome evolution is important in revealing the relationships and evolutionary processes among plant species. Moreover, it reveals the genetic foundations that ...
This analysis was the first description of the gene encoding the l subunit of AP complex in Gossypium hirsutum. In this paper, we cloned and characterized the full-length cDNA of GhAPm. Southern blot, expression patterns and structure analysis of GhAPm were also investigated. All of these ...
Tumor gene expression is predictive of patient prognosis in some cancers. However, RNA-seq and whole genome sequencing data contain not only reads from host tumor and normal tissue, but also reads from the tumor microbiome, which can be used to infer the microbial abundances in each tumor. Her...
Furthermore, the interaction between a miRNA and its target is not solely dependent on the nucleic acid pairing, but also on the availability of the target in the cell. This implies that the target gene must be transcribed, and that its secondary structure must allow the landing of the RISC...