Primer concentrations were optimized for each gene by titrating 5, 10, and 20 μM per primer. The most suitable primer concentration was chosen based on four criteria in order of decreasing importance: i) a clea
Guided by gut sensory cues, humans and animals prefer nutritive sugars over non-caloric sweeteners, but how the gut steers such preferences remains unknown. In the intestine, neuropod cells synapse with vagal neurons to convey sugar stimuli to the brain
Which single reagent can be used in the following conversions? Text Solution Which of the following reagent can be used for above conversion ? 00:57 Which reagent is used to bring about the following conversion? 02:59 Reagent used for the given conversion is : 03:00 Which of the following...
Amides are important intermediates in organic chemistry and the pharmaceutical industry, but their low reactivity requires catalysts and/or severe reaction conditions for esterification. Here, a novel approach was devised to convert amides into esters without the use of transition metals. The method effe...
Important aspects of derivatization that can be considered specific for CE analysis concern e.g., the conversion of some relevant neutral molecules such as majority of the saccharides, into derivatives that can be ionized making them suitable for CZE separations [3], [4], and the development of...
CTCs captured by this system could be used as a suitable alternative to HCC tissue detection in guiding immunotherapy, supporting the clinical application of CTC liquid biopsy.Background Hepatocellular carcinoma (HCC), one of the most common malignant tumors, has the sixth-highest incidence of all ...
Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production Christian Bogen, Arwa Al-Dilaimi, Andreas Albersmeier, Julian Wichmann, Michael Grundmann, Oliver Rupp, Kyle J Lauersen, Olga Blife...
Cells were incubated in 200 µl of fresh reduced-serum medium containing 20 µl of the CellTiter-Blue reagent for 2 h at 37 °C with 5% CO2. After incubation, 100 µl of the medium were transferred into black 96-well microtiter plates (Brand) and the fluorescence intensity was ...
13). Nevertheless, silencing neural responses using this reagent (by expression of the hyperpolarizing agent Kir2.119) produced stark deficits in hot and cold avoidance in 2-choice tests, comparable to those reported for the parental Gal4s (Fig. 1c–e and see ref. 13); we conclude that ...
Fenton Reagent The Fenton process is a viable alternative for the removal of organic pollutants from wastewater and has been applied in many industrial sectors. However, it has some disadvantages derived from the use of iron salts as a catalyst for the decomposition of hydrogen peroxide to ...