Transwell plates (8 μm pore size, Corning) were used for cell migration experiments and invasion experiments. The membranes in the upper chamber of the plates were coated with a special matrix gel (BD Biocoat, USA). After treatment with the appropriate siRNA or drugs, the cells were fixed...
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perfumes, food additives, and fine chemicals, and also provided abundant target molecules for both synthetic chemistry and synthetic biology1,13. For example, paclitaxel fromPacific yewis a first-line antitumor agent clinically used in cancer therapies14; ...
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the...
It shows the transformation of B-cell subtypes. E Box plot showing the proportions of B-LMO2 (left) and B-STMN1 (right) in MIBC, NMIBC, normal tissue, PBMC, and lymph node samples Full size image To further characterize the functions of genes enriched in the germinal center-associated B...
Next, the appropriate secondary antibody was added, and incubation was done for 1 h at room temperature. Immunoreactive protein bands were detected by using enhanced chemiluminescence reagents. Enrichment of Acetylated Peptides and Sample Preparation Four volumes of lysis buffer (8 M urea, 1% Triton...
A sample size of 3 independent experiments (biological replicates) was used for all experiments, unless otherwise noted, in order to ensure appropriate statistical power to detect a statistically significant change of at least two-fold. 3 technical replicates per biological replicate were used for ...
centering, autoscaling, pareto scaling, range scaling, vast scaling, log transformation, and power transformation, were tested on a real-life metabolomics data set. They were found to greatly affect the outcome of the data analysis and thus the rank of the, from a biological point of view, ...
The discovery of highly enantioselective catalysts and elucidating their generality face great challenges due to the complex multidimensional chemical space of asymmetric catalysis and inefficient screening methods. Here, we develop a general strategy fo
intermediate 21 or 25-acetoxyl-6-methoxy-streptovaricin C from2by StvA2 could not be accumulated to be detected owing to the efficient transformation of StvP2. In view of the above results, we reconstituted MDB biosynthetic pathway in vitro. Incubation of substrate (7) and proteins (StvM1,...