H2SO4 + MgCl2 -> 2 HCl + MgSO4 Reaction Yield: The reaction yield refers to the process of calculating the amount of product that will be obtained from a chemical reaction, considering the amount of reagents that will participate in it. Depending of the r...
The 20 nM various DNA substrate was digested by 0.2 μM protein in a final volume of 10 μl in DNA cleavage buffer (20 mM Tris–HCl, pH 9, 1 mM MgCl2 and 0.1 mg/ml BSA). Reactions were incubated at 37 °C for 5 min and then terminated by the addition of...
These values were converted to molar concentrations by using the molecular masses calculated through the automated Python script, except excluding the added mass of glycan modifications. ELISA tetramerization assays Both as a check on Bradford-based molarity calculations and for determining how to form ...
The molar mass of an element can be directly obtained from a periodic table. It is typically given in units of g/mol. For compounds however, where two or more elements are chemically combined together, it is necessary to compute the molar mass by adding up the atomic masses of each of...
The irradiated sample was dialyzed into 15 mM Tris-Cl (pH 7.5), 5 mM MgCl2, 0.1 mM EGTA, 1 mM DTT to promote filament formation of the uncross-linked myosin. After pelleting the filaments by centrifugation at 100,000 × g for 10 min, the cross-linked myosin in the supernatant was ...
Mass Percent for Solutions:In chemistry we deal with various homogeneous mixtures, which have a uniform composition containing two or more different substances. A common example is a simple solution containing one solute species dissolved in one liquid solvent species. The mass of a ...
In ethylene homopolymerization, the Cr(acac)3/MgCl2 catalyst showed almost constant activity for 3 h and produced high molecular weight polyethylene with narrow molar mass distribution.doi:10.1002/1521-3935(20000901)201:143.0.CO;2-DWILEY‐VCH Verlag GmbHMacromolecular Chemistry & Physics...
The lysate was then stir-mixed after addition of 0.2 % DDM, 20 μg/ml DNase I (20 μg/ml), 5 mM MgCl2 for 30 min, after which it was diluted to 250 ml with buffer G. Clarification of lysate was done by centrifugation at 10,000 rpm for 30 min at 4 °C and supernatant loaded...
(1.0–100 μmol/L), 5 pmol of P450 from insect microsomes, 10 pmol of purified cytochrome b5 (Oxford Bio- medical Research, Oxford, UK), and an NADPH regenerating system (1.3 mmol/L NADP+, 3.3 mmol/L glucose 6-phos- phate, 3.3 mmol/L MgCl2 and 0.4 unit/mL glucose-6-phos- phate...
Modified membrane had high salt rejection compared to unmodifed membrane, the order as: Na2SO4 > MgSO4 > NaCl > MgCl2 > LiCl. The fouling was optimally reduced when the concentration of nanocomposition on the membrane surface was increased, using humic acid as a model foulant due to dea...