Small RNAs (sRNAs) within 15-30 nt such as miRNA, tsRNA, srRNA with 3’-OH have been identified. However, whether these sRNAs are the major 15-30 nt sRNAs is still unknown. Here we show about 90% mammalian sRNAs within 15-30 nt end with 2’,3’-cyclic ph
Switchover is achieved with small interfering RNAs (siRNAs) against the endogenous RPB1 transcript and doxycycline (Dox) addition to express a stably integrated, siRNA-resistant RPB1 transgene encoding 6xHis-tagged RPB1 (Figures 1B, S1A, and S1B). Near-complete switchover was achieved, with ...
is being aggressively harnessed. Understanding the structure and function of small RNAs, such as siRNAs and miRNAs, that trigger RNAi has shed light on the RNAi machinery. In particular, it has highlighted the assembly and function of the RNA-induced silencing complex (RISC), and has provided ...
To gain insight into the complexity of small ncRNA biogenesis and function in diatoms, we combined computational and experimental analyses to generate a comprehensive characterization ofP. tricornutumsmall RNA populations. In particular, we carried out a high-throughput sequencing of short RNA fragments e...
is being aggressively harnessed. Understanding the structure and function of small RNAs, such as siRNAs and miRNAs, that trigger RNAi has shed light on the RNAi machinery. In particular, it has highlighted the assembly and function of the RNA-induced silencing complex (RISC), and has provided ...
The translation of RPs and other proteins involved in protein synthesis is regulated via a 5′TOP motif rendering the transcripts sensitive to cellular stress signals and nutrient status (Meyuhas, 2000). We show that miRNA miR-10a interacts with the 5′UTR of mRNAs encoding RPs and enhances ...
We attempted to find relationships between the methylation state in the sequences adjacent to the TE and copy number or degree of conservation within the cognate family. The individual TE families reside in different genomic segments with varying degrees of methylation, but each family is present ...
An AAS arises when an alternative 3′ splice junction (acceptor site) is used, leading to a change in the 5′ boundary of the downstream exon (Figure 1). IR occurs when a sequence is spliced out as an intron or simply retained and is distinguished from ES because introns do not flank ...
It is important to consider the biology of cancer stem cells with a particular focus on their phenotypic and metabolic plasticity, the most important signaling pathways, and non-coding RNAs (ncRNAs) regulating these cellular entities. Furthermore, the current status of therapeutic approaches against ...
With the rapid development of improved next-generation ASOs toward clinical application, this technology now holds the potential to have a dramatic effect on the treatment of many neurological conditions in the near future.This is a preview of subscription content, access via your institution Access ...