Elevated production of collagen-rich extracellular matrix is a hallmark of cancer-associated fibroblasts (CAFs) and a central driver of cancer aggressiveness. Here we find that proline, a highly abundant amino acid in collagen proteins, is newly synthesized from glutamine in CAFs to make tumour col...
Small RNAs (sRNAs) within 15-30 nt such as miRNA, tsRNA, srRNA with 3’-OH have been identified. However, whether these sRNAs are the major 15-30 nt sRNAs is still unknown. Here we show about 90% mammalian sRNAs within 15-30 nt end with 2’,3’-cyclic ph
To gain insight into the complexity of small ncRNA biogenesis and function in diatoms, we combined computational and experimental analyses to generate a comprehensive characterization ofP. tricornutumsmall RNA populations. In particular, we carried out a high-throughput sequencing of short RNA fragments e...
ALKBH5 is downregulated in GC and correlates with favorable prognosis.AThe relative expression of ALKBH5 was detected using qPCR (upper panel) or western blotting (lower panel) and normalized to GAPDH or α-tubulin.BThe relative expression of ALKBH5 in GC cell lines (SNU-216, MKN28, SGC7901...
siRNAs are the main defense guards against outside invaders, namely viruses, and represent a “cellular immune system” which is the major security line in organisms that lack an adaptive immune system (such as lower invertebrates or most plants) (Rosendo Machado et al. 2021; Kong et al. ...
Messenger RNA (mRNA) translation is tightly regulated to produce protein at the correct time and place with appropriate abundance. While many of the principles of translation regulation have been elucidated from ensemble biochemical measurements, understanding the mechanisms controlling where and when an ...
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There are several methods of designing RNAi vector libraries with regard to their targets. The first approach is to construct individual siRNAs against defined sequences of each target (usually up to four siRNA per gene), a labor-intensive process if a large number of gene targets are involved...
B. et al. Natural RNA circles function as efficient microRNA sponges. Nature 495, 384–388 (2013). This study is the first to functionally characterize naturally expressed circRNAs. Article CAS PubMed Google Scholar Izuogu, O. G. et al. Analysis of human ES cell differentiation establishes...
Protein neddylation is catalyzed by a neddylation activating enzyme (NAE, E1), an E2 conjugating enzyme, and an E3 ligase. In various types of human cancers, the neddylation pathway is abnormally activated. Our previous study validated that the neddylati