Owing to specific and compelling gene silencing, RNA interference (RNAi) is expected to become an essential approach in treating a variety of infectious, hemato-oncological, cardiovascular, and neurodegenerative conditions. The mechanism of action of small interfering RNA (siRNA) is based on post-...
Inclisiran is a PCSK9-targeting short interfering RNA (siRNA) that lowers plasma LDL-cholesterol levels.
Recent studies have highlighted the importance of the diet as a crucial factor influencing the onset and progression of RA [42,43]. The consumption of a high-fat or high-cholesterol diet can result in dyslipidaemia in RA patients, notably increasing total cholesterol (TC) and low-density lipopr...
Confirming the RNAi-mediated mechanism of action of siRNA-based cancer therapeutics in mice siRNAs that specifically silence the expression of cancer-related genes offer a therapeutic approach in oncology. However, it remains critical to determine... AD Judge,M Robbins,I Tavakoli,... - 《Journal ...
Dobson CM, Gradinger A, Longo N, Wu X, Leclerc D, Lerner-Ellis J, Lemieux M, Belair C, Watkins D, Rosenblatt DS, Gravel RA: Homozygous nonsense mutation in the MCEE gene and siRNA suppression of methylmalonyl-CoA epimerase expression: a novel cause of mild methylmalonic aciduria. Mol Gen...
Some of the small ncRNA, with a nucleotide length of less than 200, are miRNA, piwi RNA (piRNA), small interfering RNA (siRNA), and small nucleolar RNA (snoRNA) [13]. It has been noticed that piwi RNA, miRNA, and siRNA play a primary role in transcriptional gene silencing [14], [...
Number and size of neurospheres were measured as previously described [28, 29] using the software ImageJ (see supplementary methods). Neurosphere treatment and transfection Neurosphere cells were transfected with specific siRNA SmartPool One target siRNA from Horizon (Cambridge, UK) against each ...
Human ovarian cancer cell line SKOV3 and the VP16 drug-resistant cell line SKOV3/VP16 were used to investigate whether LRIG1 affects the sensitivity of SKOV3 to drugs. RT-qPCR was used to detect the difference in LRIG1 expression between drug-resistant and wild-type cell lines. siRNA LRIG...
We conducted transfection of siRNA, plasmid or siRNA + plasmid into MM1.S/BTZ and NCI-H929/BTZ cells, in accordance with the manufactory guidelines (Life Technologies, Hemel Hempstead, UK), under the application of Lipofectamine 3000. Transfection was allowed for 6–8 h, and the cells ...
Decreasing Mcl-1 alone is sufficient to induce a substantial amount of apoptosis in CLL cells in vitro: for example, when Mcl-1 expression was specifically knocked down by siRNA,46 there was a significant reduction in cell survival and enhancement of chemosensitivity. In addition, our results ...