Here we present a protocol for synthesis of small interfering RNA (siRNA) nanoparticles (NP) that target lesional macrophages as a potential treatment for atherosclerosis. Ca2+/calmodulin-dependent protein kinase γ (CaMKIIγ) activity in macrophages of advanced human and mouse atherosclerotic plaques ...
Maximal mRNA knockdown is often seen 24–48 hours after transfection, while maximal protein and phenotypic responses typically require 48–96 hours. This lag in phenotypic response is due to the fact that potent siRNAs induce a rapid reduction in target mRNA levels and...
A key component during the elongation step of protein synthesis, Ribosomal Protein LP1 forms a pentameric complex (in conjunction with LP2 and LP0) that regulates ribosome assembly and plays a role in translation initiation. Like other mammalian ribosomal proteins, Ribosomal Protein LP1 exists as ...
THE MECHANISM OF DELAYED WOUND HEALING IN THE PRESENCE OF HYPOPROTEINEMIA During the past decade protein deficiency has been shown to have an important influence on many of the problems of clinical surgery. Gastrointestinal motility, especially gastric emptying after anastomosis of the stomach and jeju...
Figure 2. siRNAs have Differential Abilities to Induce RNAi. (A) siRNAs designed to different regions of the c-myc RNA sequence were transfected into the HeLa S3 cells and analyzed 48 hr after transfection for changes in proliferation and protein levels. Cell proliferation rates were ...
In mammals and plants, cytosine DNA methylation is essential for the epigenetic repression of transposable elements and foreign DNA. In plants, DNA methylation is guided by small interfering RNAs (siRNAs) in a self-reinforcing cycle termed RNA-directed D
5. Dorsett Y, Tuschl T. siRNAs: applications in functional genomics and potential as therapeutics. Nat Rev Drug Discov. 2004;3:318–29. 6. Jeong JH, Kim SW, Park TG. Molecular design of functional polymers for gene therapy. Prog Polym Sci. 2007;32...
decreasing the expression of the target protein. The RISC complex repeatedly cleaves mRNA, thus sustaining gene silencing [51]. In contrast, in the non-productive pathway, siRNA may be sequestered into non-functional compartments, such as stress granules, processing bodies, or cytoplasmic bodies. Th...
siRNAs in targeting and degrading viral RNA has led to the development of siRNA-based treatments for diverse diseases. However, despite the promising therapeutic benefits of siRNAs, several problems, including impaired long-term protein expression, siRNA instability, off-target effects, immunological ...
The function of most miRNAs is not known; however, a number of miRNAs seem to be involved in post-transcriptional gene regulation. Some of these miRNAs (e.g. lin-4 and let-7) inhibit protein synthesis by binding to the 3' untranslated region of target mRNAs. Others bind to pe...