Lesson 1: Introduction to Basic Human Physiology Lesson 2: Physiology of Cells and Miscellaneous Tissues Lesson 3: Envelopes of the Body Lesson 4: The Skeletal System Lesson 5: Physiology and Actions of Muscles Lesson 6: The Human Digestive System Lesson 7: The Human Respiratory System...
Max Planck Institute for the Science of Human History, in duplicate by EA-IRMS on a ThermoFisher Elemental Analyzer coupled to a ThermoFisher Delta V Advantage Mass Spectrometer via a ConFloIV system. Accuracy was determined by measurements of international standard reference materials within each ana...
8A). Surprisingly, human Ri. was well customized in the mouse colon after FMT, as evidenced by its strong expression in the colon and feces of CRS + HC-tr mice (Fig. 8D). To further characterize the efficient colonization of Ri. in the mouse colon, green fluorescence-labeled Ri. ...
Finally, the labeled cells were analyzed by FCM (Becton Coulter Inc., Brea, CA, USA). Adipogenic differentiation To induce adipogenic differentiation, at 70% confluence three MSCs were respectively cultured in α-MEM supplemented with 10% FBS, 2 μm insulin, 0.5 M isobutylmethylxanthine ...
Full-length cDNAs were obtained from the Human Universal cDNA Library (HUCL) (Stratagene) by screening filters with the following 32γ-ATP end-labeled oligonucleotide: 5′-CCGGAGCTGGAGAACAACA-3′. SOST Gene Orthologs We obtained total RNA from cow (Bos taurus) and vervet monkey (Cercopithecus...
Mitochondrial DNA-deletion mutations accumulate intracellularly to detrimental levels in aged human skeletal muscle fibers Am J Hum Genet, 79 (2006), pp. 469-480 View PDFView articleCrossrefView in ScopusGoogle Scholar Cited by (53) Mitochondrial biology, targets, and drug delivery 2015, Journal ...
The Odyssey system (LI-COR) was used to develop the immunoblots with IR labeled secondary antibodies (1:30,000 dilution) for 1 h at room temperature. Monolayer dissociation of hiPSC-CMs To obtain isolated single hiPSC-CMs for intracellular Ca2+ imaging acquisition, 2D cardiac sheets were ...
In a previous study, macrophages were specifically labeled with SPIO nanoparticles and 111Indium (111In) for enhanced tracking. Inductively coupled plasma‒mass spectrometry (ICP-MS) was employed to quantify the iron content within macrophages. This approach enabled the monitoring of macrophage ...
Single-cell transcriptomics has allowed unprecedented resolution of cell types/states in the human lung, but their spatial context is less well defined. To (re)define tissue architecture of lung and airways, we profiled five proximal-to-distal locations of healthy human lungs in depth using multi...
The prioritized genes of top 20 significant fibrosis-associated DMPs are labeled. b Single-nuclear RNA-seq UMAP of 223,438 human kidney cells. Each dot represents a cell, with color coding for the cell types: Endo_GC; endothelial cells of glomerular capillary tuft, Endo_peritubular; endothelial...