The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the...
Approximately 40–50% of the total human DNA sequences are single-copy DNA sequences, meaning that about half of the human genome is composed of unique and non-repetitive sequences. According to the arrangement of repeating units, repeats can be classified into two types: tandem repeats (TRs...
Clinical archives of patient material near-exclusively consist of formalin-fixed and paraffin-embedded (FFPE) blocks. The ability to precisely characterise mutational signatures from FFPE-derived DNA has tremendous translational potential. However, seque
It has previously been estimated that any two copies of the human genome differ from one another by approximately 0.1% of nucleotide sites (that is, one variant per 1,000 bases on average) [26]. Therefore, the probability p that a given tag contains no SNP is p = (1 - 1/1000)21,...
This also applies to benign missense changes, irrespective of their allelic frequency, as clusters of BLB variants are detectable in approximately 20% of all genes associated with a hereditary condition. Based on the distribution of MutScore values in relation to protein regions and protein classes,...
Arrays of large numbers of expressed antigens have also been used (94) very effectively to define populations of autoantibodies present in human plasma. This approach improves on the classical gel Western blot by presenting all the antigens in a more native state and at approximately equal abundan...
RNA was extracted from hpCAFs (approximately 106 cells) or 2/3 cryosections of pancreatic lesion tissue using Trizol (Invitrogen) according to manufacturer's instruction. cDNA synthesis for quantitative real-time PCR (qRT-PCR) was carried out with LunaScript RT Super Mix (NEB) according to the...
Briefly, PDAC cells were seeded into 6-well plates and cultured for approximately 24 h. The culture medium was then discarded, and the cells were lysed using the NADP+/NADPH extraction reagent provided in the kit. The NADP+/NADPH ratio in the cell lysates was then measured according to the...
The dataset contains 22 patchouli accessions collected from eight localities in China, one locality in Vietnam, and ten localities in Indonesia21. The genome size of these patchouli accessions was 1226 Mb21, which is consistent with the result of our sequenced patchouli plant. The SLAF-seq ...
We synthesized human codon-optimized LSR genes and their predicted attP and attB sequences and validated recombination activity in HEK293FT human cells via a plasmid recombination assay (Fig. 2a). In this assay, the attP plasmid contains a promoterless mCherry gene, which gains a promoter upon...