but the heart weight to body weight ratio (HW/BW) of 6-month-old KO mice was significantly increased (Supplementary Fig.5g). However, starting at 9-months after birth, the KO mice exhibited lethality, while WT mice did not show abnormal viability...
(Fig.3h). We confirmed by circular dichroism that the mutant proteins did not exhibit significant structural changes compared to the wild-type enzyme, indicating that the mutations are directly responsible for the observed loss of activity with TCS-G (Supplementary Fig.S7a, b). Furthermore, ...
Four days post-challenge, the animals that ultimately recovered had slightly lower clinical scores than both the controls (p = 0.0139) and those that did not recover (p = 0.0296; RM two-way ANOVA). Non-specific pathological signs and macroscopic lesions commonly seen in animals suffering acute...
Cells prepared in this way were stored at −70°C and then used for downstream processing of scRNA-seq, flow cytometry, and CyTOF. Due to cell damage during freezing and limited cell recovery, not all samples passed QC for scRNA-seq and/or CyTOF (Table S2). Single cell RNA: Library ...
CFSE plots were analyzed by FlowJo to quantitate a “proliferation index” (total number of divisions divided by number of cells that went into division) for each condition. Results shown are an average of n = 3 T-cell donors. C, T-cells were stimulated with anti-CD3 and anti-CD28 ...
While Y318 is highly conserved in vivo, it is tolerant for mutations in the BF520 Env mutant library39. Collectively, this suggests that a Y318 bond is not critical for bnD.9 binding. Considering the negatively charged DARPin binding surface, the introduction of negative charges within V3 ...
post sham (dps) surgery and 7 days post cryoinjury (dpci), but did not observe any significant differences at these time points (Extended Data Fig.1f,g). Together these data indicate that neutrophil numbers were reduced at 24 and 96 hpci inmyd88−/−ventricles....
hederaceus (its identification number in the library is 1339), which we labeled as "SH-EAE." Applied at a concentration of 20 µg/mL, SH-EAE increased the protein expression of the UPR regulator Grp78, while it decreased the expression of IRE-1α (Figure 1), which is one of the ...