Furthermore, it is not uncommon for the files containing the data from various runs to be given the same names. The package flowFlowJo allows for this by reading in any number of FlowJo workspaces at the same time and tracking the location of the FCS files by their full pathname. Some ...
RNA passing quality control was converted to a sequencing ready library using the Agilent SureSelect Strand Specific mRNA library kit as per the manufacturer’s instructions (Agilent, Santa Clara, CA). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa ...
Cells prepared in this way were stored at −70°C and then used for downstream processing of scRNA-seq, flow cytometry, and CyTOF. Due to cell damage during freezing and limited cell recovery, not all samples passed QC for scRNA-seq and/or CyTOF (Table S2). Single cell RNA: Library ...
(Fig.3h). We confirmed by circular dichroism that the mutant proteins did not exhibit significant structural changes compared to the wild-type enzyme, indicating that the mutations are directly responsible for the observed loss of activity with TCS-G (Supplementary Fig.S7a, b). Furthermore, ...
in and 6.45 min which are assigned to the two regioisomeric In vitro kinetics. Flow cytometry experiments (representative plot Fig. 2a) in serum starved H9C2 cells exposed to log-fold varying concentrations of AlexaFFA for 2 h showed that the optimal loading concentration to load ...
Four days post-challenge, the animals that ultimately recovered had slightly lower clinical scores than both the controls (p = 0.0139) and those that did not recover (p = 0.0296; RM two-way ANOVA). Non-specific pathological signs and macroscopic lesions commonly seen in animals suffering acute...
The percentages of cells in subG1, G1/S and G2/M phases, as well as those having more than two nuclei (> 2N), were determined using the FlowJo software. For each time point, 20,000 events per sample were measured. Gates were set manually using uninduced cells, and the same ...
Our HLA class I-presented peptidome enrichment protocol did not involve the use of urea buffer, protein reduction, and alkylation or enzyme digestion. Electrospray ionization artifacts could be excluded by examining the RT of modified peptides and their unmodified counterparts. Since the artifact ...
Sample tag and cDNA libraries were generated for both the pre-treatment and on/post-treatment PBMCs (23-21712-00, BD Biosciences), while an additional TCR library was generated for on/post-treatment PBMCs (23–24018(01), BD Biosciences). Libraries were sequenced on a NovaSeq6000 flow cell...
All the data were acquired with BD influx and were analyzed with Flowjo 10.0.7. Cardiomyocytes Culture In Vitro Primary CMs were cultured in M199 medium (Gibco, Cat.No.31100035) supplemented with 0.1% bovine serum albumin (BSA, Sigma, Cat.No.B2064), 1x ITS liquid media supplement (Sigma...