While our experimental model was extensively validated and yielded a comprehensive list of PDAC-responsive substrates, it may not have captured all substrates cleaved by PDAC cells in vivo. We lysed PDAC cells via sonication, which, despite generating lysates with significant proteolytic activity, ...
FlowJo v10 provides two univariate cell cycle platforms, theWatson Pragmatic algorithm(1) andDean Jett Fox(DJF) (2). The two models differ in their mathematical calculations of each phase of the cell cycle. Consequently, results from one model may vary quite significantly from the other. It ...