we used a mouse model of diet-induced obesity (DIO) where instead of increasing NAD+by chronic supplementation with metabolic precursors, we directly supplied the metabolite itself in a daily single IP injection scheduled at ZT11, corresponding to an hour before the normal circadian rise of hepati...
The enzyme activity and the expression of DEGs were counted using Microsoft Excel 2016 and analyzed using Origin 2018 software. Data presented were means of at least three biological replicates with error bars indicating standard deviation.P < 0.05 was considered to be statistically significant. ...
resulting in female-to-maleNeratios ranging from 1.24 (scenario 2c) to 8.67 (scenario 2h) (Table2) in the scenarios without 2% intervillage male migration and without post-fission migration. These ratios were lower when considering 2% intervillage male migration but no post-fission migration (Su...
Moreover, mutations expected to be present at a 1% allelic fraction were detected across all replicates. This result indicates that sensitive detection of low allelic fraction mutations was feasible. At the time of the study, the Hong Kong and Washington viral samples were the only commercially ...
(i.e. hsa-miR-501-3p_iso_3p:-2) or with the sequence of the read itself as the counting data. This is useful since different types of modification may coexist in a single sequence, and non-templated additions and internally edited sequences can differ leading to isomiRs that can fall ...
Unique DEGs suggesting inhibition of FOXM1 included downregulation of Foxm1 itself (OCy-3). Other major biological themes that emerged from the URA and met statistical cutoff value in the OCy at 50 mg/kg included activation of TGFB1 (TGF-β) signaling and inhibition of TBX2 signaling, ...
The differential expression analysis of genes.AMean number of expressed genes of three replicates identified at each development stage.BThe number of differentially expressed genes (DEGs) for comparison of each stage with the next stage.CCluster analysis of gene expression. Embryos at different stages...
It has been reported that transcriptional noise due to DNA sequence variation can in part be buffered directly at the level of transcripts through feedback regulation onto the gene itself (Bader et al., 2015). However, such mechanisms would not explain how cells cope with residual transcript ...
(HIV) is a great example of establishing proviral latency. HIV enters the host genome as a provirus, and its proviral DNA replicates synchronously with the host. Episomal latency does not require the integration of host and viral genome and refers to the use of genetic episomes during ...
1d), suggesting that the recruitment of hCINAP to DNA-damage sites was a chronological process. The recruitment of endogenous hCINAP to the damaged sites was also detected (Supplementary Fig. 1a). We deduced that hCINAP does not function during the relatively early damage response, as it is ...