The colony formation assay was used as end point to avoid sample interference in colorimetric assays. It was possible to differentiate between four different types of MWCNTs in concentrations between 2 and 20 g/ml with this method. There was a great difference in sensitivity between the three ...
Automated methods for conducting and analyzing the colony formation assay using both the colorimetric and fluorescent properties of crystal violet.
2A, B). The results of the colony-formation assay revealed that clonogenic ability was also significantly attenuated following SOCS2-AS1 overexpression and knockdown of SOCS2-AS1 augmented EC cell-colony formation (Fig. 2C). Similarly, the 5-ethynyl-2’-deoxyuridine (EdU) proliferation assays ...
aChemical structure of NBT.bCell viability assays. The MTT assay was used to examine the cell viability in the absence or presence of different doses of NBT for 24 h.cWound healing assay. A scratch was made in monolayers of MDA-MB-231 and A549 cells, and the migration ability of the...
Read our The Mouse Colony Forming Cell (CFC) Assay using Methylcellulose-based Media to help with your experiment. Learn more.
The CCK-8 assay and the colony formation assay were performed to test cell proliferation. Briefly, for the CCK-8 assay, 1 × 103 cells were cultured in a 96-well plate at 37 °C. After 10 μl CCK-8 solution was added to each well, plates were incubated at 37 °C for...
Steele, J. G., Rowlatt, C., Sandall, J. K., Franks, L. M.Cell surface properties of high- and low-metastatic cell lines selected from a spontaneous mouse lung carcinoma.Int J Cancer.32, 769-779 (1983). Tags Soft Agar Colony Formation AssayAnchorage-independent GrowthCarcinogenesisIn Vi...
Schlunk T, Ruber E, Schleyer M (1981) Survival of human bone marrow progenitor cells after freezing: improved detection in the colony- formation assay. Cryobiology 18, 111 Article PubMed CAS Google Scholar Douay L, Gorin NC, David R, et Al (1982) Study of granulocyte-macrophage progenitor...
Colony formation assay One thousand cells were plated in 6-well plates and cultured for 10 days. The colonies were stained with 1% crystal violet after fixation with 4% formaldehyde for 15 minutes. MTS cell viability assay One thousand cells were seeded into each well in 96-well plates. At ...
the cytosolic and mitochondrial fractions were isolated by differential centrifugation as instructed and probed for cytochrome c by western blotting. In the colony formation assay, the treated cells were seeded in plates (12-well) at the indicated dilutions, grown for 10–14 days, and then stained...