At its core, the colony formation assay relies on the ability of single cells to form colonies under specific culture conditions. The principle is based on the fact that only viable and capable cells can divide and proliferate to form visible colonies, while dead or damaged cells cannot. The ...
Colony formation assayOG 1-14-2001 These assays are based on the principle that certain proteins when expressed stably cause either cell cycle arrest or cell death, hence a reduction in colony number. 1.Plate outSAOS-2(p53 -/-. pRB -/-),H1299(p53 -/-. pRB +/+) orU2OS(p53 +/+,...
Title:Soft Agar Colony Formation Assay Principle of the assay: This assay is used to measure the ability of cells to grow in an anchorage independent manner. This assay is most commonly used to assess the effect of oncogenes on anchorage independent growth or to determine if a gene is ...
Angiogenesis (Tube Formation) Assay:血管生成(管形成)的测定 热度: Colonyformationassay OG1-14-2001 Theseassaysarebasedontheprinciplethatcertainproteinswhenexpressedstablycauseeithercellcyclearrestorcelldeath,henceareductionincolonynumber. 1. PlateoutSAOS-2(p53-/-.pRB-/-),H1299(p53-/-.pRB+/+)orU2OS...
Colony formation assay on dishes To make an agarose plate, we dispensed 5 mL 2% agarose with S Medium in a 60 mm petri dish and allowed to solidify. Afterwards, we deposited worms of desired number on to the agarose plate with a pipette. We removed excess liquid and gently dispersed...
The broadly accepted colony formation assay (CFA) directly measures a cell’s ability to divide; however, it takes weeks to perform and is incompatible with high-throughput screening (HTS) technologies. Here, we describe the MicroColonyChip, which exploits microwell array technology to create an ...
The bioluminescence tests (e.g., 3M LUMAC [Biocounter, 3M Company, St Paul, MN]) are based on the principle that bacteria and mammals have distinct adenosine triphosphate (ATP) that can be destroyed selectively. After destruction of mammalian ATP, bacterial ATP can be detected by the biolumi...
As the pretreatment of marrow cells with the active principle in human sera did not show any effect on the colony growth and the coexistence of the active principle in human sera and CSF was required for the enhanced colony growth, the cooperative action of the active principle in human sera...
After an additional six weeks of proliferation, the whitefly amplification was assessed using image analysis, and then the initial viable whiteflies needed to give rise to the bio-assay quantified whitefly count was back-calculated using our previously parameterized predator-prey model for this ...
In principle, a subline of murine hemopoietic cell line NFS‐60 was cloned which was dependent on KX‐87CM. Then the growth of this subline was examined by a rapid and sensitive colorimetric tetrazolium assay. From these results, it was concluded that the CSA which KX‐87 cell line produced...