药效---靶点---验证靶点---下游通路 FIG1.Effects of apigenin on A549cell viability. 实验对象 A549 实验技术 MTT 分组 时间点:6,12,24,48h 浓度:0,20,40,60,80,100uM Colony formation assay 分组:0,20,40 key results 24h-IC50:86uM 48h-IC50:63uM FIG2.Inhibition of apigenin on migration a...
Additionally, a colony formation assay was performed to evaluate cell proliferation based on the ability of a single cell to grow into a colony through clonal expansion35, the colonies were fixed at 7 days after irradiation and stained with crystal violet (Fig.6d). We discovered that treatment ...
Colony formation assay on agarose pads Agarose pads were generated by molding melted 2% agarose (A9539-500G, Millipore-Sigma) in S Medium between two clean glass plates spaced with 1 mm-thick spacers. We used a 7 mm diameter biopsy punch (Queens Surgical) to cut out discs of agarose...
First, we used the ProLiFiler™ (Reaction Biology), a cell proliferation and survival assay with 140 human cancer cell lines, to establish the antitumor profile of ScG, its payload SN-38 and three other cytotoxins (camptothecin, MMAE and mertansine) to validate the results of our assay. ...
Crowberry suppresses the proliferation and colony formation of CCA cell lines. MTT assay was used to detect the effect of crowberry on the viability in HuCCT1 and QBC939 cells (A). Colony formation assay to detect the ability of CCA cells (B). EdU assay to detect the proliferation of CC...
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C and D, anchorage-independent growth of LIN28A knockdown and control cells was examined using a soft agar colony formation assay. Error bars, S.D. Download: Download high-res image (1MB) Download: Download full-size image FIGURE 4. LIN28A promotes cancer cell growth in vivo. A and B, ...
Colony formation assay Cells were plated in triplicate in 96-well plates at 2500 cells/well and transferred to 6-well plates 24 h post transfection. After incubation for 10–14 d at 37 °C, cells were fixed with 70 % ethanol and stained with 0.1 % Crystal Violet. The plates were then ...
Colony formation assay Colony formation studies were performed with TNBC cells on treatment with DMSO or the indicated concentrations of BAY-876. Cells were seeded into 6-well plates at 200 cells per well and left to adhere overnight. Then, cells were treated with DMSO or 3 µM BAY-876...
Colony formation assay Cells were seeded in 6-well plates, allowed to adhere overnight, and then treated with the indicated compounds. The medium was changed twice a week with fresh reagents added. After 10–14 days, the cells were fixed in 4% formaldehyde and stained with crystal violet. ...