Membrane Proteins in Vivo and in Silico: Getting the Best of Two Worldsmembrane proteinsproteomictopology predictionMembrane protein research has gained a lot of momentum in recent years: high-resolution structures are being produced at an increasing rate, membrane proteomics is coming on line, and ...
AlphaFold-Multimer63 v.3 was used for protein complex prediction when required, with default settings and relaxation, and the model with the best predicted TM score (pTM) and interface pTM score was selected. PyMol (v.2.5.0) was used to visualize selected examples. Further case-by-case ...
As expected, we found actin mostly in the cytosolic fraction, transmembrane RANK receptor in the membrane fraction, and the peripheral membrane protein Anx A5 in both fractions (Fig. 3a). While La is putatively considered a soluble protein, in differentiating osteoclasts, we found La in both ...
The Membrane Protein Data Bank (MPDB) is an online, searchable, relational database of structural and functional information on integral, anchored and peripheral membrane proteins and peptides. Data originates from the Protein Data Bank and other databases, and from the literature. Structures are ba...
We will also focus on the functions of ACBD proteins in peroxisome metabolism and at membrane contact sites. 2. Structure and evolution of the ACBD family 2.1. Structure and function of the ACB core domain The ACB domain is a phylogenetically very ancient protein structure, which is found in...
New technology platforms to identify new protein-protein interactions at the cell membrane Dualsystems the experts for protein-protein interactions at the cell membrane Dualsystems Biotech AGis specialises in elucidating protein-protein interactions in the living cells especially at the cell membrane. Dualsys...
The great advantage of this approach is the requirement of only nanogram quantities of unpurified protein. A full description of the method can be found in [39]. Finally, it is important to note that in many cases the best detergent for protein solubilization is not usually the most suitable...
Preparing Protein Lysates Lysate prep is the important first step at the beginning of a western blot experiment. Cell lysis is the breaking down of the cell membrane and the separation of proteins from the non-soluble parts of the cell. Lysate buffers contain different detergents that help to ...
Use magnetic beads for immunoprecipitation (i.e., when the sample size is < 2 mL). Magnetic beads provide the best balance of capacity/yield, reproducibility, purity, and cost savings for routine small-scale isolation of specific...
Protein-protein interaction on the cell membrane of the living cell You are here:Home New technology platforms to identify new protein-protein interactions at the cell membrane Dualsystems Biotech AGis specialises in elucidating protein-protein interactions in the living cells especially at the cell memb...