After centrifugation at 18,213 g at 4 °C for 20 min, cell lysate samples were made with 6X SDS buffer. Cell lysates were analyzed by SDS-PAGE and Western blotting. Anti-IR-pY1150/1151 (1:2000, 19H7, Cell signaling; labeled as pY IR, Cat. #3024), anti-Myc (1:2000; 9...
and those products labeled with the terms “Cultivated” and “Cultured,” were as positive as those labeled with the terms “Cell-Based,” and “Cell-Cultured.”“Cell-Cultivated” was viewed least positively. With respect to the products, the Beef...
it has historically been challenging to elucidate the cell type-specific mechanisms that promote follicular depletion and ovarian failure. Data showing age-related changes in ovarian transcriptional programs within cellular subtypes are limited, particularly those pertaining to mice. Single-cell analyses of ...
Distortion of homeostatic signaling proteins by simulated microgravity in rat hypothalamus:A(16) O/(18) O-labeled comparative integrated proteomic approach. Proteomics, 2014; 14, 262-73. doi: 10.1002/pmic.201300337 [26] Iqbal J, Li W, Hasan M, et al. Differential expression of specific ...
Briefly, PCa cells were incubated with CM-Dil-labeled exosome solution at 4 °C in the dark for 15 min. After washing three times with PBS, imaging analysis was performed using confocal laser scanning microscopy (TCS SP5, Leica). Lentivirus infection and cell transfection TcDNA3.1-based ...
An illustration of sponges, including Monorhaphis chuni (labeled 2 on the left). (Image credit: History and Art Collection/Alamy Stock Photo) Sponges are made up of colonies of animals, similar to corals, and can also live for thousands of years. Glass sponges are among the longest-living...
In addition, previous animal experiments revealed the presence of changes in cellular distribution and extracellular matrix deposition in the medial meniscus of rabbit OA models in the early disease stage [28]. In the present study, ITGB2, as an upregulated gene, was noted to be enriched in ...
The distribution of isoforms in each module (labeled with different colors) and module-trait correlation relationships is shown in Fig. 9. A number of modules displayed a close relationship with different stages. Fig. 8 Gene co-expression modules detected by WGCNA. The clustering dendrogram of ...
Each Bp cell was estimated to contain <2 pg of total RNA1,35. Single Bp cells isolated directly from host (Fig. 1a–d) were lysed, and the cDNA synthesis and amplification from single bacterium total RNA were performed1,35. The amplified cDNA was labeled with Cy3 or Cy5 dye and ...
Specific clusters for different cells types are labeled. b Violin plots showing relative expression of the top five differentially expressed (DE) genes for each cell type (color coded as in a) by comparison with all other P7 cells (gray on the right in each graph). c Violin plots for the...