Polarization of Th1/Th2 in human CD4 T cells separated by CD62L: analysis by transcription factors. Allergy 60: 780-787.S. Matsuzaki, K. Shinozaki, N. Kobayashi, K. Agematsu, Polarization of Th1/Th2 in human CD4
which controls Th2 polarization. Cloned CD4+T cells from SScskin biopsiessecrete IL-4 but not IFN-γ.94T celllines generated from SSc skin biopsies or alveolar fluid produce high levels of Th2 cytokines, and the Th2 predominance predicts accelerated decline in lung function.95,96ProteomicandDNA...
In summary, these findings indicate that BNT162b1 induces functional and proinflammatory CD4+ and CD8+ T cell responses in almost all participants, with TH1 polarization of the helper response. Discussion We observed concurrent production of neutralizing antibodies, activation of virus-specific CD4+ and...
Studies on the extended phenotype of CD21low T-bet-expressing B cells in CVID and their association with T cell subsets and immune dysregulation in CVID are warranted. Many CVID patients with immune dysregulation suffer from a combined immunodeficiency (CID), reflected by a reduction of CD4+ T...
At early timepoints during the development of colitis, HMPL-004-treated mice had significantly reduced cellularity, reduced CD4+ T cells, and IL-17+, IFN-g+, and IL-17/IFN-g double-positive cells in the spleens. These data suggest that HMPL-004 affects early T-cell proliferation and/or...
Here, we find that ZEB1 promotes MS by positively regulating Th1 and Th17 differentiation in both human and mouse CD4+ T cells. Using a mouse model of MS,experimental autoimmune encephalomyelitis (EAE), we confirm that loss of ZEB1 prevents disease onset. Mechanistically, ZEB1 controls T cell ...
Purified naive CD4 T-cells were cultured with the supernatants from BMDMs at a 1:1 ratio, in the presence of plate-coated α-CD3 (BE0001-1-1MG)/CD28 (BE0015-1-1MG, both from Bioxcell, New Hampshire, USA) antibodies at a concentration of 1 μg/mL, for 3 days. The IFN-γ+CD4+...
This study examined the influence of Th1 and Th2 helper cell subsets on atherogenesis in two ApoE−/− mouse strains, C57BL/6 and BALB/c, which display opposite T-cell subset polarizations. ApoE−/− BL/6 mice showed predominant Th1-like immune responses on polyclonal stimulation of ...
For co-cultured CD4+ cells, we used a human Th1/Th17 phenotype kit for flow cytometry according to the manufacturer’s protocol (BD Biosciences). Cells were stained for 30 min at RT with the following antibody cocktail: FITC-conjugated anti-human IFN-γ, PE-conjugated anti-human IL-17A,...
Th1 polarization Naive CD4+T cells were purified from the spleens of 7–8-week-old female mice using a CD4+CD62+T cell Isolation Kit II (Miltenyi). Mouse naive CD4+T cells were cultured at 106/ml in RPMI 1640 medium containing 10 mM Hepes, 10% fetal bovine serum, 2 mM L-glut...