JAK2STAT3TF-1细胞系目的探索JAK/STAT途径活化与细胞增殖和凋亡之间的关系.方法通过表达内源性GM-CSF受体的TF-1细胞,观察细胞增殖与凋亡;建立免疫沉淀和Western blot印迹方法,检测经GM-CSF 100 ng/ml瞬时诱导的TF-1细胞信号蛋白JAK2和STAT3酪氨酸磷酸化情况.结果经过GM-CSF刺激,细胞不但免于凋亡,TF-1细胞还呈现...
JAK2, which in turn phospho-activates STAT5 proteins at specific tyrosine residues (Tyr-694⁄699). LanthaScreenTM-STAT5 TF-1 is a human hematopoeitic cell line which constitutively expresses GFP-STAT5 fusion proteins. The JAK2⁄STAT5 signaling pathway is known to be functionally intact in...
Jak2V617F, which causes polycythemia vera and several other types of myeloid malignancies, also cannot transform Ba/F3 and 32D cells (46). The reason that Shp2E76K cannot transform Ba/F3 or 32D cells is currently unknown. However, this may be due to (a) insufficiency of Shp2E76K to ...
但JAK1和JAK2活化未改变.结论1,体内NEA模型中,HO-1抑制Th17反应,促进IL-10表达,进而抑制Th17细胞介导的中性粒细胞性气道炎症;干扰HO-1的表达,可以逆转HO-1对中性粒细胞性气道炎症的抗炎作用和对Th17细胞信号通路的抑制.2,HO-1通过阻断IL-6-STAT3-RORγt/SOCS3信号通路,抑制下游核转录因子RORγt和负反馈调节...
JAK2STAT3TF-1细胞系目的探索JAK/STAT途径活化与细胞增殖和凋亡之间的关系.方法通过表达内源性GM-CSF受体的TF-1细胞,观察细胞增殖与凋亡;建立免疫沉淀和Western blot印迹方法,检测经GM-CSF 100 ng/ml瞬时诱导的TF-1细胞信号蛋白JAK2和STAT3酪氨酸磷酸化情况.结果经过GM-CSF刺激,细胞不但免于凋亡,TF-1细胞还呈现...
(GM-R)相结合后,激活细胞内受体偶联的信号转导途径实现的,其中以JAK-STAT信号途径最为重要.然而,GM-CSF诱导相关的白血病细胞异常生物学行为与JAK-STAT途径活化之间有何关联,目前还不清楚.本研究选用GM-CSF依赖性的红白血病系细胞,观察经过GM-CSF诱导后细胞的增殖情况,以及JAK2和STAT3的活化情况,旨在探索JAK/STAT...
Modification of CellSensor irf1-bla TF-1 and irf1-bla HEL Assays for Direct Comparison of Wild-Type JAK2 and JAK2 V617F InhibitioningentaconnectAssay & Drug Development Technologies
与对照组比较,人参皂苷Rgl作用后TF-1细胞EPOR总蛋白的表达水平无明显变化,但降低了TF-1细胞对EPO的反应性,磷酸化EPOR的表达水平明显下调,Bax和caspase-3(激活型)蛋白的表达水平明显上调,Bcl-2以及EPOR下游JAK2,磷酸化.IAK2,STAT5和磷酸化STAT5的表达水平均明显下调(P均〈0.05).结论:人参皂苷Rgl可明显抑制TF-1...