JAK2STAT3TF-1细胞系目的探索JAK/STAT途径活化与细胞增殖和凋亡之间的关系.方法通过表达内源性GM-CSF受体的TF-1细胞,观察细胞增殖与凋亡;建立免疫沉淀和Western blot印迹方法,检测经GM-CSF 100 ng/ml瞬时诱导的TF-1细胞信号蛋白JAK2和STAT3酪氨酸磷酸化情况.结果经过GM-CSF刺激,细胞不但免于凋亡,TF-1细胞还呈现...
如下图所示，TF可通过激活JAK2-STAT5通路导致癌细胞不受控制地生长，继而阻止癌细胞凋亡；也可通过激活RTKs促进肿瘤细胞的生长；TF还可通过增加VEGF、抑制TSP导致肿瘤细胞的血管生成和转移增加；另外，TF可以激活rac1，rac1可进一步活化PKA从而调控MAPK，导致癌细胞的生长和转移；TF亦可激活PAR2，PAR2又通过多种方式导...
又如对于一个试验体系,如IL-6的试验体系,该体系牵涉到将细胞同与可溶性IL-6受体连接的试剂接触,该细胞表达gp130β-组分和诸如Jak1、Jak2或Tyk2的信号转导组分。 在本发明的另一实施方案中,用以上途径建立了一种筛选方法,用以筛选在配体结合、受体复合物形成及接下来的信号转导过程的各步中起作用的小分子拮抗剂...
盐酸杰克替尼是公司自主研发的一种新型JAK抑制剂类药物,属于1类新药,公司拥有该产品的自主知识产权。杰克替尼对Janus激酶包括JAK1、JAK2、JAK3和TYK2具有显著的抑制作用,且对JAK2和TYK2的抑制作用最强。另外,杰克替尼还可以通过抑制激活素受体1(ACVR1)活性降低铁调素转录,改善铁代谢失衡,增加血红蛋白,降低骨髓纤维化患...
positive[37]. Similarly, Ding et al. showed that MAFG-AS1/miR-3196/TFAP2Aaxis is implicated in the malignant progression of breast cancer and elevating glycolysis levels through activating JAK2/STAT3 signaling pathway[38]. According to a recent study, the mechanism of MAFG-AS1 in enhanced ...
BY-2168R-RBITC Anti-ADAR1 (C-terminus)/RBITC 罗丹明(RBITC)标记的双链RNA腺苷酸脱氨基酶抗体(C端) 进口/国产BY-1353R-HRP Anti-BECN1/Beclin 1/ATG6/HRP 辣根过氧化物酶标记的Bcl-2同源结构域蛋白抗体 进口/国产BY-3206R-HRP Anti-phospho-JAK2(Tyr221)/HRP 辣根过氧化物酶标记的磷酸化蛋白酪氨酸...
"IL1RN" "IL2" "IL21" "IL2RA" "IL6" "IL6" "IRF1" "JAK2" "JAK3" "JUNB" [73] "KLF11" "KRT17" "LCAT" "LEP" "LGALS3BP" "LTBP1" "MCL1" "MCL1" "MDC1" "MICA" "MMP1" "MMP14" [85] "MMP2" "MMP2" "MMP3" "MMP7" "MMP7" "MMP9" "MMP9" "MUC1" "MUC4" "MYC"...
JAK2, which in turn phospho-activates STAT5 proteins at specific tyrosine residues (Tyr-694⁄699). LanthaScreenTM-STAT5 TF-1 is a human hematopoeitic cell line which constitutively expresses GFP-STAT5 fusion proteins. The JAK2⁄STAT5 signaling pathway is known to be functionally intact in...
8 标准品 0.5ml×1瓶 9 标准品稀释液 1.5ml×1瓶 10 封板膜 2张 试剂和样本的控制方法: 一、试剂 1.试剂的选择:国家明确要求要采用批批检定的形式对ELISA试剂严格把关,我司严格按照*进行 ,已获得国家承认的“三证”,每一个产品都有对应的 批号,只要客户提前下单,我们就能为您提供新批次的产品,不必担心会...
Jak2V617F, which causes polycythemia vera and several other types of myeloid malignancies, also cannot transform Ba/F3 and 32D cells (46). The reason that Shp2E76K cannot transform Ba/F3 or 32D cells is currently unknown. However, this may be due to (a) insufficiency of Shp2E76K to ...