tetramer staining 读音: 美 英 tetramer staining基本解释 四聚体染色 分词解释 tetramer四聚物 staining着色v. (使)染色( stain的现在分词 ) 猜你喜欢 fluorescent staining荧光染色(法) negative staining负染色法 staining method染色方法 staining technique染色技术 acridine orange staining吖啶橙荧光染色 alcian ...
To aid detection of low frequency populations that are beyond the limit of detection of standard direct Class II tetramer staining, expansion [1, 2] or enrichment with magnetic beads [3, 4] may be required; although some very low affinity antigens may not give detectable staining. ...
网络释义 1. 四聚体染色 四聚体染色(tetramer-staining)技术是最近几年发展起来的灵敏度最高、操作相对比较简单的抗原特异性CTL检测方法。T细胞识 … cdmd.cnki.com.cn|基于 1 个网页
In situ tetramer staining (ISTMHC class I tetramersCD8+ T cellsvibratomeconfocal microscopyWith the onset of MHC tetramer technology came a wealth of new understanding of antigen specific CD8 + T cells. This chapter discusses the application of MHC tetramer technology to stain antigen specific T ...
MHC四聚体染色(MHC Tetramer Staining)技术广泛用于抗原特异T细胞的检测和分析。Tetramer Shop开发的新一代MHC四聚体系列产品,可以在30分钟内快速完成四聚体的批量制备。通过双方的战略合作,宸安生物的解决方案可以支持用户同时进行T细胞抗原表位高通量筛选和T细胞多参数“表型+功能”分析,并且相关产品均可通过荧光标记来...
3. 制备 2X Staining Cocktail:FACS Buffer+ MHC 四聚体(建议稀释比例为 1:50～1:100)+ Anti-CD8/FITC 抗体或其他用作内参的抗体(请参考其说明书进行稀释)。 染色孵育 4. 取 25ul 2X Staining Cocktail,滴加入步骤 2 中的微孔培养板或 FACS 试管,与细胞悬浮液混匀。(注意:使用移液器轻柔上下吹打混匀,...
MHCclassIItetramerstaining(mouse) Needed: -Roundbottom96wellplate -Culturemedium(CM): Iscove’smodifiedDulbecco’smediumsupplementedwith10% fetalcalfserum,0.5mMb-mercapto-ethanol,penicillin(100 U/ml)andstreptomycin(100mg/ml) -MHCclassIItetramers ...
Non-Invasive:MHC-peptide tetramer staining is a non-invasive technique, it relies on flow cytometry or fluorescence microscopy to identify T cells binding to the tetramer. Quantification:This technique allows quantification of T cells, providing an accurate measure of the frequency of antigen-specific...
The development of MHC tetramer staining has opened the doors to multiple avenues of new research [Science 274 (1996) 94]. In this review, we will discuss the development and application of in situ MHC tetramer (IST) staining. We describe two independently developed IST staining methodologies an...
n. 四聚物;四聚体 tetramer是什么意思 网络 四聚体法; 与四聚体法; 四聚体复合物词组短语 1.tetramer technology 四聚体技术 2.Tetramer staining 四聚体染色 3.glycine tetramer 甘氨酸四聚物 4.peptide tetramer 肽四聚体技术 5.G-tetramer 四聚体 6.MHC-peptide tetramer 肽四聚体技术 7.aniline tet...