This is the principle behind biosensor systems such as the Biacore® or the ProteOn® system. They can also be used to determine the dissociation constant KD by using the following formula: K D = [ K off ] [ K on ] As used herein, the term pharmaceutical composition refers to a ...
Table S2. Mass Cytometry Staining Panels and Tetramers Used, Related to STAR Methods Table S3. Broad Immune Cell Analysis, Related to Figures 1, 2 and 3 Table S4. Cytokine Luminex Analysis, Related to Figures 1 and S1 Table S6. Analysis that Compares Immune Parameters from the Acute and Ea...
Gel electrophoresis and staining with silver was done as described (10). Analytical Gel Filtration The molecular mass of MspA was determined by analytical gel filtration. A silica-based (G3000SWXL, Tosoh Biosep) and dextran-based material (Superdex 200, Pharmacia) were used for gel filtration ...
Adding a monomer purification principle based upon affinity-tagged peptides, we here provide a robust method to produce class II tetramers and demonstrate staining of antigen-specific CD4+ T cells. We also provide evidence that both MHC class II and T cell receptor molecules largely accept ...
In principle, decrease in fluorescence quenching or less accessibility of Trp residues to acrylamide (Trp protection) would be expected if unfolded (monomeric) KcsA refolds back into tetramer upon TFE dilution. The percent reversibility values, calculated from acrylamide quenching constants, are complied...
A population of TTCF tetramer-positive B cells was identified in all analyzed donors, whereas background staining with the control tetramer was very low (Figure 3B). TTCF tetramer-positive B cells were brightly labeled, with an intensity of more than 1 log above background for the majority ...
Isotype indicates staining with an isotype control antibody. (C) IFN-γ production by A05 and A01 T cell clone in the presence of AM Ac2SGL and transfected C1R cells. WT, wild type. Controls are shown as white bars and CD1b mutants are shown as black bars. Data are representative of ...
When more than 75% of the exiting peptide is exchanged with a target peptide, the resulting tetramer can be used for CD8 + T cell staining. An exchange rate of higher than 90% corresponds to binding affinities in the low nanomolar range, while exchange rates lower than 65% are indicative ...