In this study we developed a TEM-1 尾-lactamase based protein translocation assay and applied this assay for identification ofT4SS effectors. Anhypothetical protein, APH0215 is identified as a T4SS effector protein and found interacting with-Golgi network in transfected cells. Hereby, this protein...
β-lactamase fusionCCF4FluorescenceLegionella pneumophilaAmong the bacterial secretion systems, the Type III, IV, and VI secretion systems enable bacteria to secrete proteins directly into a target cell. This specific form of secretion, referred to as translocation, is essential for a number of ...