Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD 490 nm), which...
open the β-lactam ring, moving the two fluorophores apart and disrupting the FRET. This is measured as a change in fluorescence of substrate molecules from green to blue and only requires the presence of 100 molecules of the β-lactamase reporter, making it an incredibly sensitive assay. The...
说明书 技术支持 货号PV3575 CNY5,427.00 10 µg 下单后预计有货时间 07-Apr-2025 查看价格 批量或定制请求 产品信息 应用 建议稀释比 已发表文章 Kinase Assay (KA) Assay-dependent - 产品规格 种属 Human Expression System E. coli 分子量 38 kDa ...
Additional applications derived from coupling this novel assay method to methods in the art include: a method for specific detection of microbial beta-lactamases; a method for simultaneous detection of microbial cytochrome c oxidase systems and penicillinases, and its application to simultaneous ...
Molecular / Cellular Chromogenic substrates, such as nitrocefin, are frequently used to assay the activity of beta-lactamase or measure its inhibition Payne et al (1994). The change in absorbance of various beta-lactam substrates in the course of hydrolysis is commonly used Christensen et al (19...
Addition of 5 mM cyclic AMP (cAMP) or 0.5 mM cAMP-N6, O2-dioctanoyl (a lipophilic derivative) resulted in a marked drop of β-lactamase induction by imipenem as compared to the control assay. Monitoring of carbapenem hydrolysis by cell-free supernatants revealed inactivation of both ...
Beta-Lactamase Activity Assay Kit β-Lactamase Activity Assay Kit 基本信息 NACRES NA.84 General description【一般描述】 β-内酰胺酶(bL; EC 3.5.2.6)最初从大肠杆菌中鉴定出来的,已被称为青霉素酶。随后,已经从各种细菌中鉴定出许多βL。βL能够特异性水解抗生素所含的β-内酰胺环,如青霉素、头孢菌素、...
All these plant extracts were further checked for their inhibition of Extended Spectrum Beta-lactamase in the screening assay. Eight of the plant extracts showed potentiation of cephalosporins against the Extended Spectrum Beta-lactamase producing Klebsiella pneumoniae strain and were checked for their ...
J. Iodometric assay of penicillinase. Nature 1954, 174: 1012–1013. PubMed Google Scholar Novick, R. P. Analysis by transduction of mutations affecting penicillinase formation inStaphylococcus aureus. Journal of General Microbiology 1963, 33: 121–136. PubMed Google Scholar Hovelius, B., ...
We developed a whole-cell biosensor that quantifies beta-lactamase inhibition in a cellular context. This assay accounts for factors such as membrane permeability and can be used to identify novel beta-lactamase inhibitors. 展开 关键词: IN-VITRO ACTIVITY PSEUDOMONAS-AERUGINOSA CARBAPENEMASE NXL104 ...