Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD 490 nm), which...
Beta-Lactamase Activity Assay Kit β-Lactamase Activity Assay Kit 基本信息 NACRES NA.84 General description【一般描述】 β-内酰胺酶(bL; EC 3.5.2.6)最初从大肠杆菌中鉴定出来的,已被称为青霉素酶。随后,已经从各种细菌中鉴定出许多βL。βL能够特异性水解抗生素所含的β-内酰胺环,如青霉素、头孢菌素、...
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货号PV3575 10 µg CNY5,196.00 查看价格 下单后预计有货时间 15-Jan-2025 数量 批量或定制请求 性能保障 说明书 技术支持 产品信息 应用 建议稀释比 已发表文章 Kinase Assay (KA) Assay-dependent - 产品规格 种属 Human Expression System E. coli ...
open the β-lactam ring, moving the two fluorophores apart and disrupting the FRET. This is measured as a change in fluorescence of substrate molecules from green to blue and only requires the presence of 100 molecules of the β-lactamase reporter, making it an incredibly sensitive assay. The...
Beta-lactamase inhibitory potential of fifteen plants was checked by beta-lactamase enzyme inhibition assay wherein inhibition of commercial beta-lactamase by their extracts was determined using a chromogenic substrate. These plant extracts showed considerable beta-lactamase inhibition. All these plant ...
Cloning assay showed that CAE-1 confers resistance to ampicillin, piperacillin, cefazolin, cefuroxime, and ceftriaxone, and elevates the MIC of ampicillin-sulbactam two-fold in Escherichia coli DH5α, suggesting that CAE-1 functions as a broad-spectrum β-lactamase. Amino acid sequences analysis ...
We developed a whole-cell biosensor that quantifies beta-lactamase inhibition in a cellular context. This assay accounts for factors such as membrane permeability and can be used to identify novel beta-lactamase inhibitors. 展开 关键词: IN-VITRO ACTIVITY PSEUDOMONAS-AERUGINOSA CARBAPENEMASE NXL104 ...
Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae. APMIS 2007, 115, 1400–1408. [Google Scholar] [CrossRef] Coculescu, B.; Palade, A.; Delcaru, C.; Coculescu, E.C. Genetic analysis of multidrug-resistant Salmonella enterica ...
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