In this study we developed a TEM-1 尾-lactamase based protein translocation assay and applied this assay for identification ofT4SS effectors. Anhypothetical protein, APH0215 is identified as a T4SS effector protein and found interacting with-Golgi network in transfected cells. Hereby, this protein...
Translational fusion of an effector with a reporter protein of known activity remains the best method to monitor effector translocation. The development of a fluorescent substrate for the TEM-1 beta-lactamase has turned this antibiotic-resistant protein into a highly versatile reporter system for ...