(TALENs) are examples of modified nucleases that are based on protein-DNA interactions and can target DNA in a sequence-specific manner for DSB purposes [31]. These proteins are engineered to recognize and bind DNA via covalent interactions between effector domains and short nucleotide sequences, ...
Here, we review single-cell sequencing techniques for individual and multiomics profiling in single cells. We mainly describe single-cell genomic, epigenomic, and transcriptomic methods, and examples of their applications. For the integration of multilay
especially when coupled with Laser Capture Microdissection (LCM) (Fig.2b, c). NGS is often referred to as massively parallel sequencing. Various NGS techniques enable the determination of nucleotide sequences in
A composite features-based model is proposed by integrating three different sample representation methods i.e., Dinucleotide Composition (DNC), Trinucleotide Composition (TNC) and Tetranucleotide Composition (TetraNC) for precise splice site prediction after converting the DNA sequences into numerical ...
reading frame) via translational repression [106,107]. Therefore, engineering uORFs by genome editing can also be an effective approach for crop improvement [108]. In certain cases, it is easier to upregulate gene expression by altering the uORF than by changing the coding sequences and ...
CRISPR-Cas9 technology operates by guiding RNA molecules to locate and directly cut or modify the DNA sequence. This enables gene regulation through gene knockout as well as genome editing, including gene insertion or base substitution. Genome editing of hepatocytes is extensively employed to model ...
Clinical application of liquid biopsy in gastric cancer (GC). Circulating tumor cells (CTCs), circulating free DNA (cfDNA), circulating tumor DNA (ctDNA), non-coding RNAs (ncRNAs), exosomes, and proteins in the blood of GC patients can be used as potential biomarkers for liquid biopsies and...
The most stable structure of DNA is the canonical right-handed double helix termed B DNA. However, certain environments and sequence motifs favor alternative conformations, termed non-canonical secondary structures. The roles of DNA and RNA secondary str
CRISPR-Cas9 technology operates by guiding RNA molecules to locate and directly cut or modify the DNA sequence. This enables gene regulation through gene knockout as well as genome editing, including gene insertion or base substitution. Genome editing of hepatocytes is extensively employed to model ...
DiCleave: a deep learning model for predicting human Dicer cleavage sites Lixuan Mu Jiangning Song Tomoya Mori BMC Bioinformatics(2024) Distinguishing word identity and sequence context in DNA language models Melissa Sanabria Jonas Hirsch Anna R. Poetsch ...