The association between tyrosine-phosphorylated SLP-76 and Vav seems to be required for NF-kB activation in response to coengagement of CD3 and CD28.8 In addition to its involvement in TCR- induced NF-kB activation, Vav is also important for NFAT, AP1, JNK activation and for sustained Ca2 ...
JR3.11-transduced cells were transfected with a luciferase reporter plasmid containing 3 tandem copies of the composite NFAT/AP1-response element from the human IL-2 gene promoter, together with a pRL-CMV Renilla reporter construct (Promega, Madison, WI), as described.34 After 24 hours, cells ...