BMC Biology 2014, 12:32 http://www.biomedcentral.com/1741-7007/12/32 RESEARCH ARTICLE Open Access Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile Catherine Reynolds1, Deborah Chong1, Eleanor Raynsford1, Kathryn Quigley1, Deborah Kelly1, Julia Llewellyn-Hughes2, Daniel ...
BMC Biology 2014, 12:32 http://www.biomedcentral.com/1741-7007/12/32 RESEARCH ARTICLE Open Access Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile Catherine Reynolds1, Deborah Chong1, Eleanor Raynsford1, Kathryn Quigley1, Deborah Kelly1, Julia Llewellyn-Hughes2, Daniel ...
alpha-Chain T-Cell Antigen Receptor基因重排α链T细胞抗原受体Porcine T-cell receptor alpha (TCRalpha)-chain cDNA clones were isolated from libraries made from two different sources, the thymus of a 1-month-old LW strain pig and the peripheral blood lymphocytes (PBL) of a 5-month-old Clawn ...
Learn about our SMARTer approach to single T-cell sequencing that obtains alpha-beta pairing information.
由于特定的TCR alpha-beta链配对介导抗原特异性,获得配对信息对于深入了解抗原识别,靶向免疫治疗TCR的有效设计至关重要,也有助于确立T细胞群的祖先关系。单个T细胞测序可以确定特定细胞的配对信息,但是价格可能非常昂贵并涉及复杂的分析,因为大部分方法需要对大量的细胞测序。
Analysis of T cell antigen receptor (TCR) expression by human peripheral blood CD4-8- alpha/beta T cells demonstrates preferential use of several V beta genes and an invariant TCR alpha chain. J Exp Med 1993; 178: 1–16. 12 Tilloy F, Treiner E, Park SH, Garcia C, Lemonnier F, de ...
The SMARTer Human TCR a/b Profiling Kit enables users to analyze T-cell receptor (TCR) diversity from single T cells that have been sorted into a 96-well plate. As the name suggests, the kit can be used to generate data for both alpha- and beta-chain diversity. T...
Deep learning predictions of TCR-epitope interactions reveal epitope-specific chains in dual alpha T cells Abstract T cells have the ability to eliminate infected and cancer cells and play an essential role in cancer immunotherapy. T cell activation is elicited by the binding of the T cell ...
(ii) wherein the alpha chain variable domain CDR3 length varies among the plurality of displayed TCRs and the beta chain variable domain CDR3 length varies among the plurality of displayed TCRs, wherein screening comprises: a) panning the library using as a target the pHLA antigen; b) ...
length, (as found in natural repertoires) is preferable. A substantial portion of any library is generally lost to stop codons, frame shifts, folding problems and TCR chain combinations that could simply never bind to an HLA complex. Taking into account the huge number of variable alpha and ...