Step 1: Acetyl CoA (two carbon) combines with oxaloacetate (4 carbon) to form citrate (6 car...
Full size image Next, we analyzed PGK1 expression levels using a panel of colon cancer cell lines and two normal colon cell lines NCM460 and CRYPT (Fig. 1d). All of the seven colon cancer cell lines displayed higher PGK1 expression levels compared to the normal cell lines, which was cons...
Download: Download full-size image Figure 5. Stimulatory effect of TCA metabolites is eukaryotic specific Citrate and succinate were titrated from 0 to 40 mM in 5 mM increments and added to decatenation assays with HsTop2B (A–D), HsTop2A (E–H), and Ec topo IV (I–L), and to super...
The next step in the GABA shunt involves oxidation of SSA by SSA dehydrogenase to form succinate (Renault et al., 2010). Succinate is utilized in TCA cycle and as an electron donor for the electron transport chain. GABA metabolism has been connected with carbon–nitrogen (C/N) balance and...
In a functional TCA cycle (Figure 2, reactions 1 to 13), the presence of this anaplerotic pathway depends on two enzymes: isocitrate lyase, ICL (EC: 4.1.3.1), and malate synthase (EC: 2.3.3.9) (Figure 2, reactions 14 and 15). The full glyoxylate cycle is a combination of glyoxylate...
In a functional TCA cycle (Figure 2, reactions 1 to 13), the presence of this anaplerotic pathway depends on two enzymes: isocitrate lyase, ICL (EC: 4.1.3.1), and malate synthase (EC: 2.3.3.9) (Figure 2, reactions 14 and 15). The full glyoxylate cycle is a combination of glyoxylate...