该课题组的前期工作发现并命名了一个脊椎生物特有的自噬调控分子Pacer,调控自噬体成熟和脂代谢。(详见BioArt报道:Mol Cell丨浙大孙启明组揭示自噬体成熟和脂代谢调控的新机制)【1, 2】。对其作用机制进行深入研究,发现RAB2A和TBC1D4都是P...
WesternECL 试剂盒购自CellSignaling 公司。 1.3 原核表达质粒的构建 根据载体多克隆酶切 位点及TBC1D4 的序列 ,设计引物。 引物由上海生 工生物科技有限公司合成 ,引物的序列为 :P15′ CGCGGATCCATGGAGCCGCCCAGCTGCAT;P25′GCC CTCGAGTGGCTTATTTCCTATCTTGG。 从健康体检者 外周血单个核细胞(peripheralbloodmono...
Ploug, T., Van Deurs, B., Ai, H., Cushman, S. W. & Ralston, E. Analysis of GLUT4 distribution in whole skeletal muscle fibers: Identification of distinct storage compartments that are recruited by insulin and muscle contractions. J. Cell Biol. 142, 1429–1446 (1998). Article CAS Pub...
TBC1D4 encodes the TBC1D4/AS160 RabGTPase activating protein (RabGAP) involved in the translocation of glucose transporter 4 (GLUT4) from the cytosol to the cell membrane. Although the precise functional mechanism underlying insulin resistance in the proband is yet to be determined, this case ...
phosphorylation of AS160 and TBC1D1 plays an important role for GLUT4 mobilization to the cell surface. The phosphorylation of AS160 and TBC1D1 in humans i... MH Vendelbo,AB Moller,JT Treebak,... - 《Journal of Applied Physiology》 被引量: 22发表: 2014年 RabGAPs TBC1D1 and TBC1D...
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A., An, D., Toyoda, T., Koh, H. J., et al. (2010). Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle. Am. J. Physiol. Cell Physiol. 298, C377-C385. doi: 10.1152/ajpcell.00297.2009...