the use of shorter probes. For more information on MGB probes, please consult the following manual: "Primer Express v1.5 and TaqMan MGB Probes for Allelic Discrimination: All PCR Instruments: User Bulletin." You can find a copy on our website by entering this title in the main search box....
For more information on MGB probes, please consult the following manual: "Primer Express v1.5 and TaqMan MGB Probes for Allelic Discrimination: All PCR Instruments: User Bulletin." You can find a copy on our website by entering this title in the main search box. ...
Gene expression is highly controlled and regulated in living cells. One of the first steps in gene transcription is recognition of the promoter site by the TATA box Binding Protein (TBP). TBP recruits other transcriptions factors and eventually the RNA polymerase II to transcribe the DNA in mRNA...
Mutated TATA-box/TATA binding protein complementation system for regulated transgene expression in tobacco. Plant J 2007;50:917-25.Chaturvedi CP, Lodhi N, Ansari SA, Tiwari S, Srivastava R, Sawant SV, Tuli R (2007) Mutated TATA-box/TATA binding protein complementa- tion system for regulated...
Even in the presence of an upstream light response activator element, TATA-box mutations influenced modulation of the promoter by light. An A at the eighth position was specifically involved in the red light response of the promoter. Selectivity in gene expression was associated with a high level...
Box 1.6 Promoter-bashing experiments help identify the importance of specific promoter sequences in regulating gene expression. These experiments make use of deletion mutations to narrow down the region of interest; then individual bases are mutated to define the core functional sequence involved in reg...
In addition to the TATA box, GC box and poly (A) tail, other gene expression-related elements composed of variant nucleotide sequences could also be targets of BBR in biological systems. Due to the limited cell-compartment distribution of BBR and the different binding affinities of BBR to ade...
Protein–protein interaction assays using purified proteins revealed that residues 124–220 of the EICP27 protein mediate its direct interaction with TATA box-binding protein (TBP). Partial deletion of this TBP-binding domain attenuated the ability of the EICP27 protein to stimulate the IE and ...
The TATA-box-binding protein (TBP) of Halobacterium salinarum - Cloning of the tbp gene, heterologous production of TBP and folding of TBP into a native co... The TATA protein () is a critical factor used for nucleating assembly of the polymerase II machinery. binds TATA box elements with...
TAF7L (TATA-Box Binding Protein Associated Factor 7 Like) is similar to a mouse gene that encodes a TATA box binding protein-associated factor, and shows testis-specific expression. The encoded protein could be a spermatogenesis-specific component of the DNA-binding general transcription factor comp...