The design of proteins that bind to a specific site on the surface of a target protein using no information other than the three-dimensional structure of the target remains a challenge1,2,3,4,5. Here we describe a general solution to this problem that starts with a broad exploration of th...
Members of the ATP-binding cassette (ABC)-F protein subfamily collectively mediate resistance to a broader range of clinically important antibiotic classes than any other group of resistance proteins and are widespread in pathogenic bacteria. Following over 25 years' of controversy regarding the mechanis...
These off-target interactions can be categorized into two types: hybridization-dependent and hybridization-independent. The former arises when ASOs bind to unintended RNAs in a sequence-dependent manner. Conversely, the latter originates from non-specific interactions between ASOs and proteins, facilitated...
Mammalian target of rapamycin complex 2 modulates αβTCRB processing and surface expression during thymocyte development Mammalian target of rapamycin complex 2 modulates alphabetaTCR processing and surface expression during thymocyte development. The Journal of Immunology, 193, ... Ruegg,A M.,Chou,....
Based on the protein–protein docking simulations of selected protein models of Klotho with Wnt3 and Wnt3a, different peptides derived from Klotho have been designed. Wnt3 and Wnt3a proteins have three important domains: Index finger, N-terminal domain and a patch of ∼10 residues on the ...
Molecular regulation of target genes by AphA has been characterized in Vibrio harveyi and V. cholerae, but it is still poorly understood in V. parahaemolyticus. Methodology/Principal Findings The AphA proteins are extremely conserved in V. parahaemolyticus, Vibrio sp. Ex25, Vibrio sp. EJY3, V. ...
Yang Xin Tang (YXT) is a traditional Chinese herbal preparation which has been reported to improve cognitive function and memory in patients with dementia. As the underlying mechanism of action of YXT has not been elucidated, we examined the effects of YXT and its major herbal components in reg...
While in HeLa cell extracts these proteins are complexed with hUpf1, in intact cells hUpf3a and hUpf3b are nucleocytoplasmic shuttling proteins, hUpf2 is perinuclear, and hUpf1 cytoplasmic. hUpf3a and hUpf3b associate selectively with spliced β-globin mRNA in vivo, and tethering of any h...
In this context, the utrophin gene can produce two full-length isoforms, utrophin A and utrophin B, which are transcribed from distinct promoters and have different 5′-untranslated regions (5′-UTRs)16,28. Both proteins are identical, except for N-terminal regions28. The 5′UTR of utrophin...
Fig. 1: IS110 mobile genetic elements express a ncRNA that is bound by its encoded recombinase. a, Schematic representation of the IS110 recombinase protein sequence. b, Schematic representation of the structure and life cycle of an IS110 element. Core sequences are depicted as green diamonds,...