TaqMan PCR Protocol Author: SC, Patti Lab, 3/03 Real-time quantitative RT-PCR (Taqman)This is performed as a 2-step reaction:1. cDNA synthesis from DNase 1-treated total RNA 2. PCR 1. cDNA synthesis (Advantage™ RT-for-PCR Kit - Clontech)All reagents listed are provided with the...
Real-Time RT-PCR (Taqman®) of Tumor mRNA to Predict Sensitivity of Specimens to 5-FluorouracilProtocol pp 257–265 Cite this protocol Chemosensitivity: Volume II Tetsuro Kubota Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 111)) 844 Accesses Abstract 5-...
In this work, primers and TaqMan probes were designed, and duplex and multiplex real-time quantitative PCR (qPCR) protocols were developed and optimized for the simultaneous quantification of R. solanacearum and their phages. The quantification range was established from 108 to 10 PFU/mL for th...
当赛默飞预设计的即用型TaqMan Assay无法满足您的实时荧光定量PCR研究需求时,TaqMan探针和qPCR引物成为您的不二之选。赛默飞提供一系列双标记TaqMan探针和用于qPCR引物的非标记寡核苷酸,研究人员可以根据各种实验需求灵活地选择合适的探针和引物。研究人员无论是使用定制探针通过生物信息学技术同时检...
Protocol: TaqMan® Gold RT-PCR Kit: Protocol (English ) Brochures & Specifications: TaqMan® Gold RT PCR Kit: Product Bulletin (English ) Brochures & Specifications: TaqMan® Gold RT-PCR Kit: Real-Time PCR Reagents: Product Bulletin (English ) ...
A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of IsolatesProtocol pp 55–71 Cite this protocol Hepatitis C Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J. M. Alexander & Martin D. Curran Part of the book series: Methods in Molecular ...
Material & Methods: A total of 251 pharyangeal and nasal swabs from suspected cases of swineflu were processed by TaqMan real-time PCR (CDC protocol). Clinical co-relation with presenting sign and symptoms and analysis was done. Results: 36 (14%) were confirmed as positive. The clinical ...
TaqMan Universal 预混液 II 将执行实时聚合酶链反应 (PCR) 所必需的组分(引物、探针、模板和水除外)混合在一起,十分便利。在多种应用,包括定量和基因分型,可将 TaqMan Universal 预混液 II 用于扩增互补 DNA (cDNA) 和 DNA 靶标。 这种TaqMan Universal 预混液 II 作为一种2X 浓缩物提供,并含有: ...
W. 2009. Example of use of TaqMan real-time RT-PCR to analyze bacterial gene transcript levels: Haemophilus influenzae. Curr. Pro- toc. Microbiol. 1:Unit 1D1.Johnston JW. 2009. Example of use of TaqMan real-time RT-PCR to analyze bacterial gene transcript levels: Haemophilus influenzae . ...
Real-time PCR was first described using hydrolysis probes (Heid et al., 1996; Gibson et al., 1996), often referred to as the TaqMan system. The technique is based on the coupling of two important processes: first, the construction of dual-labeled oligonucleotide probes, also called hydrolysis...