采用免疫沉淀技术检测调节CB2R对TAK1和TRAF6之间相互作用的影响以及TAK1泛素化水平.结果:免疫荧光染色显示小鼠脊髓损伤后1周损伤部位的CB2R表达量显著增加,CB2R在脊髓损伤后的差异性表达在小胶质细胞中最显著.BMS评分,水箱实验和步态实验结果表明JWH-133促进小鼠脊髓损伤后的运动功能恢复.免疫荧光染色和ELISA结果显示...
NF-r。B 信号转导通路与T AK I激酶在通路中的功能中文摘要转化生长因子激酶1(TAKl)是丝裂源激活蛋白激酶激酶激酶( MAP3K )家族成员之一, 在功能上位于丝裂源蛋白激酶( MAPK )和Ir,.B 激酶的上游。 在体内可以既可以被包括IL- 1在内的多种细胞因子激活, 参与机体的许多重要生理过程, 又可以通过与结合...
遗传学专业优秀论文 NF-kB信号转导通路与TAK1激酶在通路中的功能 关键词:免疫遗传 蛋白激酶 信号转导 摘要:转化生长因子激酶1(TAK1)是丝裂源激活蛋白激酶激酶激酶(MAP3K)家族成员之一,在功能上位于丝裂源蛋白激酶(MAPK)和IKB激酶的上游。在体内可以既可以被包括IL-1在内的多种细胞因子激活,参与机体的许多重要生...
JNK和p38信号通路激活所必需的.野生型TAK1的转入,可以恢复基因敲除细胞内IL-1通路的完整性.同时证明TRAF6也是通过TAK1激活NFkB途径.TAK1不但可以诱导IKKb的磷酸化,并且在NEMO多泛素化修饰过程中也是必不可少的.本研究证实Thr178和Thr184是两个可调节性磷酸化位点,它们的磷酸化在TAK1/TAB1共转染及IL-1诱导的...
sitesofthisMAP3K.AndthepreciseregulationforTAKlactivationatthemolecularlevelisstillnotfllllyuncovered.HerewereportthatdualphosphorylationofThrl78andThrl84residueswithinthekinaseactivationloopisessentialforTAKl-mediatedNF l出a ndhL廿b activation.SubstitutionofthesetworesidueswithalanineabolishedtheabilityofTAKlto...
By overexpression and RNAi knockdown, we showed that inducible HSP70 modulated the NF-kB but not MAPKs signalings through influencing the stability of TAK1 protein in HeLa cells. Moreover, overexpression of HSP70 attenuated the production of iNOS upon IL-1β stimulation, validating that inducible ...
TAK1 inhibition attenuates NF-kB activation in VSMCs.Zifang, SongXiaolei, ZhuRong, JinCuiping, WangJinchuan, YanQichang, ZhengAnil, NandaD., Neil GrangerGuohong, Li
2006. Small interfering RNAs generated by recombinant dicer induce inflammatory gene expression in- dependent from the TAK1-NFkB-MAPK signaling pathways. Biochem. Biophys. Res. Commun. 347: 566-573.
TAK1 inhibition blocks vascular injury-induced phosphorylation of both TAK1 and NF-kB in the vessel wall.Zifang SongXiaolei ZhuRong JinCuiping WangJinchuan YanQichang ZhengAnil NandaD. Neil GrangerGuohong Li
TAK1 ubiquitination regulates doxorubicin-induced NF-kB activation. Cell. Signal. 25: 247-254.Liang L, Fan Y, Cheng J, Cheng D, Zhao Y, Cao B, Ma L, An L, Jia W, Su X, Yang J, Zhang H. TAK1 ubiquitination regulates doxorubicin-in- duced NF-kappaB activation. Cell Signal 2013;...