TOPO® TA Cloning® kits for sequencing provide a highly efficient, 5-minute cloning reaction for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites ...
The TOPO™ TA Cloning™ Kits for Sequencing provide a highly efficient, 5-minute, one-step cloning strategy ('TOPO™ Cloning') for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO™ TA vector with ...
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with speci...
We have removed much of the multiple cloning site from the pCR™4-TOPO® TA vector to shorten the distance between sequencing primer sites and the insert site to as little as 33 bp. This means sequencing reactions give less vector sequence and more insert sequence. The pCR™4-TOPO®...
TOPO TA Cloning for sequencing TOPO TA Fast cloning (with Mach1™ Competent Cells) 图1. TOPO克隆步骤。 TOPO PCR克隆只需三个简单的步骤。 将PCR产物与TOPO克隆载体混合,等待五分钟,然后转化入大肠杆菌。 步骤 Topo TA 克隆试剂盒 TA/UA 克隆 限制性酶切连接法 使用现有引物? 是 是 否 载体是否可...
4.1.2Optional Procedure: TA Cloning of DNA Libraries and Sequencing As an optional step, DNA libraries prior to selection can be cloned into plasmids byTA cloningmethods as is done following selection. Though not essential, this practice can provide useful control data, establishing whether there ...
测序用 TOPO™ TA Cloning™ 试剂盒可提供高效、5 分钟的一步克隆策略(“TOPO™ 克隆”),用于将 Taq 聚合酶扩增 PCR 产物直接插入质粒载体中以进行测序。每个试剂盒均使用含经过特殊设计的测序引物位点(可从各反应中获得更多的插入序列和更少的载体序列)的 pCR™4-TOPO™ TA 载体。这些试剂盒具有克隆和...
测序用 TOPO™ TA Cloning™ 试剂盒可提供高效、5 分钟的一步克隆策略(“TOPO™ 克隆”),用于将 Taq 聚合酶扩增 PCR 产物直接插入质粒载体中以进行测序。每个试剂盒均使用含经过特殊设计的测序引物位点(可从各反应中获得更多的插入序列和更少的载体序列)的 pCR™4-TOPO™ TA 载体。这些试剂盒具有克隆和...
pMD18-TVector是一种高效克隆PCR产物(TACloning)的专用载体。本载体由pUC18载体改建而成,在pUC18载体的多克隆位点处的Xbal和Sall识别位点之间插入了EcoRV识别位点,用EcoRV进行酶切反应后,再在两侧的3’端添加“T”而成。因大部分耐热性DNA聚合酶进行PCR反应时都有在PCR产物的3’末端添加一个“A”的特性,所以使用...
Cost-Effective TA Cloning Applied to Sanger Sequencing and HLA Allele TypingNEDUVAT, Anupama CheleriMURTHY, Prerana MadhusudhanaSUNDARRAJAN, SudarsonPADMANABHAN*, SriramWalailak Journal of Science & Technology