In this study, the TA-GC cloning method is proposed, as a new, simple and efficient method for the directional cloning of protein-coding genes in expression vectors. The presented method features several advantages over existing methods, which tend to be relatively more labour intensive, ...
(tandem) sequences in automatedDNA sequencingruns due to difficulty in separating the distinct base pairs and leading to useless information. Other similar cloning approaches include the GC Cloning kit technology (Lucigen Inc., Madison, WI, USA) that is based on the enzymatic addition of a ...
5. NPY, CART and CCK identification in winter flounder ( Pleuronectes americanus) and winter skate (Raja ocellata ): Molecular cloning, tissue distribution and response to season and fasting. [D] . MacDonald, Erin E. 2008 机译:冬季比目鱼(Pleuronectes americanus)和冬季滑冰(Raja ocellata)...
亚克隆用 TOPO™ TA Cloning™ 试剂盒可提供高效、5 分钟的一步克隆策略(“TOPO™ 克隆”),用于将 Taq 聚合酶–扩增 PCR 产物直接插入一个质粒载体上以进行亚克隆。每个试剂盒使用含便利限制性位点的 pCR™ 2.1-TOPO™ TA 载体进行亚克隆。根据您的需要和预算,您可选择含多种感受态细胞或不含感受态...
To control the complex biosynthetic machinery, precise understanding of biosynthetic genes and enzymes is expected to be necessary. Herein, we describe the cloning and sequencing of the FD-594 biosynthetic gene cluster from the producer Streptomyces sp. TA-0256 and propose a plausible biosynthetic ...
TA Cloning™ 试剂盒提供多种配置:含 One Shot™ INVF' 化学感受态大肠杆菌(K2050-01 和 K2050-40)、含 One Shot™ TOP10F' 化学感受态大肠杆菌(K2060-01 和 K2060-40)以及不含感受态细胞(K2750-20 和 K2750-40),分 20 和 40 次反应试剂盒规格。
The Mighty TA-Cloning Kit allows fast, easy TA cloning of PCR products with 3’ A-overhangs, as generated withTaq-based DNA polymerases. It includes the DNA Ligation Kit <Mighty Mix>, which enables highly efficient ligations to be performed simply and quickly. ...
Asgardarchaeota have been proposed as the closest living relatives to eukaryotes, and a total of 72 metagenome-assembled genomes (MAGs) representing six primary lineages in this archaeal phylum have thus far been described. These organisms are predicted
Cloning of WEI8 reveals a prevalent role of this route and implicates local auxin production in plant growth and development. To reflect the biochemical nature of the protein defective in wei8 and in an allelic mutant, sav3, described in the accompanying paper by Tao et al. (2008), we ...
产品应用• Molecular cloning • Restriction site mapping • Genotyping • Southern blotting • Restriction fragment length polymorphism (RFLP) • SNP Note: For methylation sensitivity, refer to product specifications. •分子克隆 •限制性位点比对 •基因分型 •Southern印迹 •限制性片段长...