亚克隆用 TOPO™ TA Cloning™ 试剂盒可提供高效、5 分钟的一步克隆策略(“TOPO™ 克隆”),用于将 Taq 聚合酶–扩增 PCR 产物直接插入一个质粒载体上以进行亚克隆。每个试剂盒使用含便利限制性位点的 pCR™ 2.1-TOPO™ TA 载体进行亚克隆。根据您的需要和预算,您可选择含多种感受态细胞或不含感受态...
Li JL, Tang BZ, Hou YM, Xie YX (2016) Molecular cloning and expression of the vitellogenin gene and its correlation with ovarian development in an invasive pestOctodonta nipaeon different two host plants. Bull Entomol Res 106. doi:10.1017/S0007485316000353 ...
Small subunit rRNA gene in silico survey The SSU rRNA gene survey was based on the SILVA SSU database (release 132, Ref NR 99)16 (https://www.arb-silva.de/). We extracted the habitat information (field ‘habitat_slv’, ‘isolation_source’ and ‘lat_lon’ in SILVA ARB database) and...
test (n = 108 and 90 wheat accessions;P = 2.168 × 10−7). Brown lines, upper and lower quartiles; green lines, medians.gThe expression ofTaWD40-4B.1in the accessions harboringTaWD40-4B.1CandTaWD40-4B.1Thaplotypes under the well-watered and water-withheld conditions ...
In insects, the widely known function of CTLs is the activation of immune signalling pathways (Li et al., 2015; Zhu et al., 2016; Brown et al., 2018), such as converting prophenoloxidase into phenoloxidase or influencing the expression of the prophenoloxidase gene directly (Yu et al., ...
2. Hara I.et al., 1995.Implicating a role for immune recognition of self in tumor rejection: passive immunization against the brown locus protein. J Exp Med. 182(5):1609-14. 3. Nimmerjahn F. & Ravetch J.V., 2005.Divergent immunoglobulin g subclass activity through selective Fc receptor...
A significant antimutagenic activity was found in the hot water-soluble extract from a common edible brown alga, Laminaria japonica (Makonbu in Japanese) which showed suppressive effects on umu gene expression of the SOS response against DNA damage in Salmonella typhimurium (TA1535/pSK1002). The ...
Brown, Priscilla N.Argani, PedramAshfaq, RaheelaFukushima, NoriyoshiTaylor & FrancisCancer Biology & TherapyHustinx SR, Hruban RH, Leoni LM, et al. Homozygous deletion of the MTAP gene in invasive adenocarcinoma of the pancreas and in peri- ampullary cancer: a potential new target for therapy....
We cloned a gene encoding β-glucuronidase (sGUS) that is involved in the initiation of HOmetabolism in skullcap (). This gene consists of a 1581-nucleotid... Sasaki,K. - 《Journal of Biological Chemistry》 被引量: 96发表: 2000年 Identification and Cloning of gusA, Encoding a New -Glucu...
We have modified current methods to create a very efficient technique for cloning cDNAs in a defined orientation, into plasmid vectors bearing phage SP6 an... NH Brown,FC Kafatos - 《Journal of Molecular Biology》 被引量: 1354发表: 1988年 The small nucleolar RNAs The present review...