Small non-coding RNAs(sRNAs)have received much attention in recent years due to their unique biological properties,which can efficiently and specifically tune target gene expressions in bacteria.Inspired by natural sRNAs,recent works have proposed the use of artificial sRNAs(asRNAs)as genetic tools ...
Efficient cell-free protein expression from linear DNA templates has remained a challenge primarily due to template degradation. In addition, the yields of transcription in cell-free systems lag behind transcriptional efficiency of live cells. Most commonly used in vitro translation systems utilize T7 ...
(Graumann & Premstaller, 2006). The T7 promoter is incorporated into theexpression vectorsuch that it controls the transcription of the heterologous gene; the T7 promoter is only recognized byT7 RNA polymeraseand, thus, the gene coding for T7 RNA polymerase is introduced into thebacterial ...
A schematic of the promoter DNA is shown (below) with the specific binding region –17 to –5 highlighted in gray, identifying the initially melted bubble region (gray oval) and noting the 3′-thiol modification. MATERIALS AND METHODS Mutant Construction—An expression vector coding for the ...
Foot-and-mouth diseasePhage displayVP1 epitopeFoot-and-mouth disease (FMD) is a major threat to the livestock industry worldwide. Despite constant surveillance and effective vaccination, the perpetual mutations of the foot-and-mouth disease virus (FMDV) pose a huge challenge to FMD diagnosis. The...