Efficient cell-free protein expression from linear DNA templates has remained a challenge primarily due to template degradation. In addition, the yields of transcription in cell-free systems lag behind transcriptional efficiency of live cells. Most commonly used in vitro translation systems utilize T7 ...
(Graumann & Premstaller, 2006). The T7 promoter is incorporated into theexpression vectorsuch that it controls the transcription of the heterologous gene; the T7 promoter is only recognized byT7 RNA polymeraseand, thus, the gene coding for T7 RNA polymerase is introduced into thebacterial ...
Furthermore, 80% (4/5) of T7-GH immunized pigs were protected from challenge with virulent homologous virus. These findings demonstrate that the T7-GH phage nanoparticles were effective in eliciting antigen specific immune responses in pigs, highlighting the value of such an approach in the ...
Cross-linking of promoter DNA to any of the native cysteines would allow the RNA polymerase to survive the high salt challenge, but function with DNA would almost certainly happen in trans, as it should not allow correct positioning of the promoter on the protein to which it is cross-linked...