A series of T7 promoters described previously [1, 2, 11, 12], see Table 1 for sequences, were placed upstream of the broccoli coding sequence via primer extension. Sense strand primers with promoter sequences, the first 23 nucleotides of the broccoli coding sequence, and the anti-sense pr...
(Graumann & Premstaller, 2006). The T7 promoter is incorporated into theexpression vectorsuch that it controls the transcription of the heterologous gene; the T7 promoter is only recognized byT7 RNA polymeraseand, thus, the gene coding for T7 RNA polymerase is introduced into thebacterial ...
Cross-linking of promoter DNA to any of the native cysteines would allow the RNA polymerase to survive the high salt challenge, but function with DNA would almost certainly happen in trans, as it should not allow correct positioning of the promoter on the protein to which it is cross-linked...