(i) membrane complex : 由 TssJ,TssL,TssM(TssJLM)组成,锚定在内膜上并与外膜相连; (ii)baseplate complex(BP):TssE,TssF,TssG,TssK 和 VgrG构成(PAAR); (iii)The tail tube/sheath complex (TTC):由三聚体 VgrG-PAAR 穿刺装置顶端的六聚体 Hcp 环组成,内部管道外是由 TssB/C 组成的尾管/鞘结构...
总之,我们提供了进一步的T6SS并显示,一个噬菌体sheathlike的结构很可能是一个保守的元素在所有T6SSs的结构和机制的深入了解。 原文摘要: The HsiB1C1 (TssB-TssC) Complex of thePseudomonas aeruginosa Type VI Secretion System Forms a Bacteriophage Tail Sheathlike Structure Protein secretion systems in Gram-...
Here, we experimentally confirm the identity of this T6SS and, by cryo electron microscopy (cryoEM), show the structure of its post-contraction sheath at 3.7 angstrom resolution. We demonstrate the assembly of this T6SS by IglA/IglB and secretion of its putative effector proteins in response ...
The cyto- solic part of the T6SS docks onto a membrane complex (TssLMJ) probably by interacting with a phage baseplate-like structure (Durand et al., 2015; Filloux and Freemont, 2016; Planamente et al., 2016). On contraction of the TssBC sheath, the T6SS effectors are propelled out ...
The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI secretion system forms a bacteriophage tail sheathlike structure J Biol Chem, 288 (11) (2013), pp. 7536-7548 View PDFView articleView in ScopusGoogle Scholar Lu et al., 2013 D. Lu, Shang G, Q. Yu, H. Zhang, ...
6 The contraction of an engulfing sheath structure propels the needle and effectors out of the cell.6,7 T6SS effectors come in two flavors: (1) specialized effectors, which are the secreted structural proteins Hcp, VgrG, and PAAR carrying a C-terminal toxic domain extension, and (2) ...
Kudryashev M, Wang RYR, Brackmann M, Scherer S, Maier T, Baker D et al (2015) Structure of the type VI secretion system contractile sheath. Cell 160:952–962 ArticlePubMed CentralGoogle Scholar Lertpiriyapong K, Gamazon ER, Feng Y, Park DS, Pang J, Botka G, Graffam ME, Ge Z,...
the tail tube sheath complex: 细胞穿刺装置类似于可收缩的噬菌体尾部结构。T6SS的内管由Hcp六聚体组成,它是一种与T4噬菌体尾管蛋白gp19具有相似结构的蛋白质。同时 Hcp 六聚体的堆叠需要 VgrG 和周围 TssBC 鞘的组装。 ①六个TssBC二聚体形成一条链,包裹一个Hcp六聚体环。TssBC二聚体可分为三个区域:...
T6SS biogenesis starts with the assembly of the membrane complex (MC), followed by recruitment of the baseplate complex (BC), which serves as the tail assembly platform for polymerization of the tail tube/sheath structure [6,7]. The MC is composed of an outer membrane lipoprotein TssJ and ...