C. (2014). Assembly and structure of the T3SS. Biochim. Biophys. Acta 1843, 1649-1663. doi: 10.1016/j.bbamcr.2014.01.035Burkinshaw BJ, Strynadka NC (2014) Assembly and structure of the T3SS. Biochim Biophys Acta 1843:1649-1663
Overall, the structure revealed unique interactions between the various interfaces of AopB and AcrH, with the N-terminal “molecular anchor” of AopB crossing into the “N-terminal arm” of AcrH. AopB adopts a novel fold, and its transmembrane regions form two pairs of helical hairpins. ...
The core structure is the ~4 MDa needle complex (NC), built on a foundation of three highly oligomerized ring-forming proteins that create a hollow scaffold spanning the bacterial inner membrane (IM) (24-mer ring-forming proteins PrgH and PrgK in the Salmonella enterica serovar Typhimurium...
Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both ...
Many bacteria move using a complex, self-assembling nanomachine, the bacterial flagellum. Biosynthesis of the flagellum depends on a flagellar-specific type III secretion system (T3SS), a protein export machine homologous to the export machinery of the v
reveal a striking colocalization of a T3SS-encoding locus, its transcriptional activators, protein products, and the complete structure at the cell membrane, which they claim provides evidence for a mechanism known as 'transertion'.doi:10.1038/s41467-023-37753-wI. Fishov...
Structure 23, 161-172.Bergeron J. R. et al. . The modular structure of the inner-membrane ring component PrgK facilitates assembly of the type III secretion system basal body . Structure 23 , 161–172 (2015).Bergeron J R,WorraU L J, De S, et al.The modular structure of the inner...
The structure of Spa33-C2 and identification of an unexpected intramolecular pseudodimer in Spa33-FL reveal a molecular model for their higher order assembly within NF-T3SS. Spa33-FL and Spa33-C are identified as functional counterparts of a FliM-FliN fusion and free FliN respectively. Further...
This cocoon is thicker than any described cellular actin structure and functions as a gatekeeper for the cytosolic access of the pathogen. Host CDC42, TOCA-1, N-WASP, WIP, the Arp2/3 complex, cortactin, coronin, and cofilin are recruited to the actin cocoon. They are subverted by T3SS ...
Fig. 3. Topology and structure of the BAM complex. The cartoon summarizes what is known about the architecture of the BAM complex, derived through the results of genetics and molecular interactions. Shown are the crystal structures available for each subunit of the BAM complex ordered according to...