Quick Tips - Why is there precipitate in my T4 DNA ligase buffer? 观看其他视频 库存 货号浓度规格目录价北京上海广州成都苏州武汉 B0202S10 X5 ml¥409有有有有有有 如何订购 Need a custom/large volume order?Contact us 产品信息 NEB 公司的每种酶都提供 10X 反应缓冲液。在 1X 浓度时,该反应缓冲...
T4 DNA Ligase Reaction Buffer B0202SVIAL -20 1 x 1 ml 10 X 特性和用法 单位定义 1 单位是指在 20 µl 反应体系,1X T4 DNA 连接酶反应缓冲液中,16℃ 条件下 30 分钟内能使 50% 的经 HindIII 消化的 λ DNA 片段 [DNA 5´ 末端浓度为 0.12 µM (300 μg/ml)] 连接所需的酶量。
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not...
To purify and remove inhibitors, extract the DNA with buffer-saturated phenol, then extract with chloroform:isoamyl alcohol, and precipitate with ammonium acetate and ethanol. Be sure that the DNA is free of phenol and that the phosphate concentration is less than 25 mM and the NaCl concentration...
The performance of the product is not affected provided that the precipitate is resuspended.Enzyme Storage Buffer: T4 DNA Ligase is supplied in 10mM Tris-HCl (pH 7.4), 50mM KCl, 1mM DTT, 0.1mM EDTA and50% glycerol.Source: E. coli strain expressing a recombinant clone.Unit Definition: 0.01...
T4 DNA LigaseM0202MVIAL-201 x 0.05 ml2,000,000 units/ml T4 DNA Ligase Reaction BufferB0202SVIAL-201 x 1 ml10 X 特性和用法 单位定义 1 单位是指在 20 µl 反应体系,1X T4 DNA 连接酶反应缓冲液中,16℃ 条件下 30 分钟内能使 50% 的经 HindIII 消化的 λ DNA 片段 [DNA 5´ 末端浓...
isinsolution(typically1–2minutes).Theperformanceoftheproduct is not affected provided that the precipitate is resuspended. Enzyme Storage Buffer: T4 DNA Ligase is supplied in 10mM Tris-HCl (pH 7.4), 50mM KCl, 1mM DTT, 0.1mM EDTA and
DNA的酶切与连接——DNA片段连接 实验方法原理核酸片段可以通过连接酶的作用连接起来而获得重组分子。实验材料λDNA EcoT14I:由11条DNA片段组成试剂、试剂盒T4 DNA连接酶及其配套的10×连接缓冲液 0.5×TBE电泳缓冲液 6×Loading Buffer Goldview 琼脂糖仪器、耗材电泳仪 水浴锅 移液器 微波 2019-03-25 16:25 ...
• Since T4 Polynucleotide Kinase is inhibited by ammonium ions, use sodium acetate to precipitate DNA prior to phosphorylation. For Research Use Only. Not for use in diagnostic procedures. 規格 Concentration10 U/μL EnzymeT4 Polynucleotide Kinase Compatible Buffer10X Reaction Buffer Quantity2,500 ...
T4 PNK can label nucleic acids with 5′-phosphate in the exchange reaction using reaction buffer B. However, for efficient labeling we recommend to remove the 5′-phosphate groups of DNA/RNA (e.g. by using Thermo Scientific FastAP, Cat. Nos. EF0651/2/4) and then phosphorylate in the ...