之前研究表明T1R1的Venus flytrap(VFT)结构域主要负责鲜味配体的识别.在本研究中,以T1 R1-VFT作为识别元件,通过Au-S键将其自组装在双金属纳米材料(MoS2-PtPd)上,构建了一种用于检测鲜味物质的仿生受体传感器.该传感器在检测不同种类鲜味物质(氨基酸,核苷酸,有机酸,鲜味肽)时均有良好的线性关系,检测限低至0.03pM...
分子对接结果表明,鲜味肽主要通过氢键与鲜味受体T1R1-VFT结合,关键作用位点包括149THR,151ARG和108ASP等.通过分子动力学模拟与MM/PBSA结合自由能计算,发现鲜味肽与鲜味受体的结合产生了更稳定的复合物体系,其中带正电荷的氨基酸对总结合自由能产生了积极的贡献.本研究为更好地理解鲜味物质与鲜味受体之间的相互作用提供...
鲜味受体T1R1T1R1-VFT鲜味分子互作特性构象变化鲜味受体(Umami taste receptor type 1 member 1/3,T1R1/T1R3)异二聚体亚基T1R1胞外捕蝇草结构域(T1R1-Venus flytrap domain,T1R1-VFT)是主要的配体结合区域.然而,T1R1-VFT/鲜味配体的关键结合过程仍未系统解析.本研究通过异源表达体系制备了足量,功能活性的T1 R1...
umami electrochemical biosensorT1R1 Venus flytrap (VFT)AuNPs@ZIF-8/Ti3C2 MXeneimmobilizationThe bioelectronic tongues based on human taste receptor and nanomaterials sensing platforms have been emerging with human-like taste perception. However, the reLiu, JingZhang, Ninglong...
T1R1 Venus flytrap (VFT)GrapheneField-effect transistor (FET)Numerous efforts have been made to measure tastes for various purposes. However, most taste information is still obtained by human sensory evaluation. It is difficult to quantify a degree of taste or establish taste standard. Although ...
To develop the T1R1-VFT biosensor using the above-mentioned method, we utilized the ligand binding domain of the human umami receptor, rather than the entire umami receptor protein, which had a complex structure, having the following advantages: volume reduction, simplicity, and stability. This ...