NMDS ordination of TRFLP fingerprints.Zohar, PasternakAshraf, AlAshhabJoao, GaticaRon, GafnyShlomit, AvrahamDror, MinzOsnat, GillorEdouard, Jurkevitch
How to get more out of molecular fingerprints: practical tools for microbial ecology single-strand conformation polymorphism (SSCP), length heterogeneity-PCR (LH-PCR), terminal-restriction fragment length polymorphism (tRFLP) and 16S rRNA gene... M Marzorati,L Wittebolle,N Boon,... - 《...
(b) little variability between the four replicates of the same soil, (c) the patterns of the two brown soils were more similar to each other than to those of the other two soils, and (d) the fingerprints of the different soil types revealed significant differences in a permutation test,...
After amplification, the PCR product is then digested with restriction enzymes such as HhaI, MspI, and RsaI to generate T-RFLP fingerprints of bacterial communities. In the present study, a mixture of dairy and horse manure (dairy+horse manure; 1:1 ratio w/w) was composted in windrows ...
T-RFLP profiling is a very effective method for comparing many samples in an environmental microbiology study, because fingerprints of microbial diversity can be generated in a sensitive,...doi:10.1007/978-1-62703-712-9_5Catherine A Osborne...
However, the routine application of such functional fingerprints needs careful method development and evaluation. This study aimed at identifying a reliable, highly resolving fingerprinting method to monitor alkane degrading communities in soil. Therefore, we (i) evaluated various available terminal ...
DNA fingerprintsFunctional diversityGenetic profilesMicrobial community analysesMicrobial diversitySingle-strand conformation polymorphism (SSCPStructural diversityTerminal restriction fragment length polymorphism (T-RFLPSingle-strand conformation polymorphism (SSCP) and terminal restriction fragment length polymorphism (T...
Using an AMF-specific, 18s rDNA-based terminal restriction fragment length polymorphism (T-RFLP) analysis method, significant differences were found between AMF community fingerprints of samples derived from roots of grasses with (G Cm ) and without (G 0 ) neighboring C. maculosa . There were ...