NMDS ordination of TRFLP fingerprints.Zohar, PasternakAshraf, AlAshhabJoao, GaticaRon, GafnyShlomit, AvrahamDror, MinzOsnat, GillorEdouard, Jurkevitch
Terminal restriction fragment length polymorphism (T-RFLP) analysis is a popular high-throughput fingerprinting technique used to monitor changes in the structure and composition of microbial communities. This approach is widely used because it offers a compromise between the information gained and labor ...
complexity of genetic fingerprints, T-RFLP analysis has three main advantages as compared to other DNA based techniques (Marsh, 1999): (i) T-RFLP technol- ogy with capillary electrophoresis has considerably higher resolution than the commonly used gel electro- phoretic systems; (ii) quantity and...
After amplification, the PCR product is then digested with restriction enzymes such as HhaI, MspI, and RsaI to generate T-RFLP fingerprints of bacterial communities. In the present study, a mixture of dairy and horse manure (dairy+horse manure; 1:1 ratio w/w) was composted in windrows ...
We found that the composition of the TRFLP fingerprints varied by different extraction procedure. The best quality and quantity of community DNA extracted from fecal material was obtained by using the QIAamp DNA stool minikit (Qiagen, Valencia, CA) with 95掳C incubation and moderate bead beating...
However, the routine application of such functional fingerprints needs careful method development and evaluation. This study aimed at identifying a reliable, highly resolving fingerprinting method to monitor alkane degrading communities in soil. Therefore, we (i) evaluated various available terminal ...
Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP FingerprintsMolecular characterization of Phytophthora isolates from tobacco, tomato, carnation, gerbera, crossandra and periwinkle was...
DNA fingerprintsFunctional diversityGenetic profilesMicrobial community analysesMicrobial diversitySingle-strand conformation polymorphism (SSCPStructural diversityTerminal restriction fragment length polymorphism (T-RFLPSingle-strand conformation polymorphism (SSCP) and terminal restriction fragment length polymorphism (T...
Using an AMF-specific, 18s rDNA-based terminal restriction fragment length polymorphism (T-RFLP) analysis method, significant differences were found between AMF community fingerprints of samples derived from roots of grasses with (G Cm ) and without (G 0 ) neighboring C. maculosa . There were ...